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Principaux documents

PKH26PCL

Sigma-Aldrich

PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling

Distributed for Phanos Technologies

Synonyme(s) :

Phagocytic cell label

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32

Application

This kit is for phagocytic cell labeling. It is used to selectively label cells with phagocytic capabilities such as monocytes, macrophages or neutrophils.

Principe

The labeling occurs through the formation of dye aggregates or particulates. The aggregate formation significantly inhibits the uptake of dye by non-phagocytic cells, such as lymphocytes, but facilitates dye uptake by phagocytic cells. Labeled cells appear patchy or spotted because the dye is localized in phagocytic compartments of the cells. The dye appears to be resistant to metabolic attack and has been found to remain with the cells for at least 21 days in vivo.
Labeling of phagocytic cells by this methodology may be conducted either in vitro or in vivo. Intraperitoneal or intravenous injections of the PKH26 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.

Liaison

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Informations légales

CellVue is a registered trademark of Phanos Technologies

Composants de kit seuls

Réf. du produit
Description

  • Diluent B 6 x 10

  • PKH26 cell linker in ethanol .5 mL

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

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Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Irrit. 2 - Flam. Liq. 2

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

57.2 °F - closed cup

Point d'éclair (°C)

14.0 °C - closed cup


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Consulter la Bibliothèque de documents

Jeffrey H Jennings et al.
American journal of respiratory cell and molecular biology, 32(2), 108-117 (2004-11-26)
Apoptotic cells must be cleared to resolve inflammation, but few resident alveolar macrophages (AMo) from normal lungs ingest apoptotic cells. We examined how Mo ingestion of apoptotic cells is altered during immune inflammation induced by intratracheal challenge of primed C57BL/6
Hiroshi Kanno et al.
International journal of molecular sciences, 14(5), 9604-9617 (2013-05-07)
A specialized population of cells residing in the hair follicle is quiescent but shows pluripotency for differentiating into epithelial-mesenchymal lineage cells. Therefore, such cells are hoped to be useful as implantable donor cells for regenerative therapy. Recently, it was reported
U Maus et al.
American journal of physiology. Lung cellular and molecular physiology, 280(1), L58-L68 (2001-01-03)
The evaluation of monocytes recruited into the alveolar space under both physiological and inflammatory conditions is hampered by difficulties in discriminating these cells from resident alveolar macrophages (rAMs). Using the intravenous injected fluorescent dye PKH26, which accumulated in rAMs without
Solène Accarias et al.
Innate immunity, 22(5), 382-392 (2016-05-26)
Resident macrophages play a central role in maintaining tissue homeostasis and immune surveillance. Here, we used single cell-based qPCR coupled with flow cytometry analysis to further define the phenotypes of large and small resident peritoneal macrophages (LPMs and SPMs, respectively)
Sung Hoon Baik et al.
Neurobiology of aging, 35(6), 1286-1292 (2014-02-04)
Immune responses in the brain are thought to play a role in disorders of the central nervous system, but an understanding of the process underlying how immune cells get into the brain and their fate there remains unclear. In this

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