MINI67
PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling
Distributed for Phanos Technologies
Synonyme(s) :
Green PKH membrane mini labeling kit
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32
Produits recommandés
Conditionnement
pkg of 1 kit
Niveau de qualité
Fabricant/nom de marque
Distributed for Phanos Technologies
Conditions de stockage
protect from light
Technique(s)
flow cytometry: suitable
Fluorescence
λex 490 nm; λem 502 nm (PKH67 dye)
Application(s)
cell analysis
detection
Méthode de détection
fluorometric
Conditions d'expédition
ambient
Température de stockage
room temp
Description générale
PKH67 kit is for general cell membrane labeling. It has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Application
PKH67 green fluorescent cell linker mini kit for general cell membrane labeling has been used to stain:
- exosomes
- pericytes
- UT-7/TPO cells
This kit is for general cell membrane labeling. Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes.
Liaison
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Informations légales
Distributed for Phanos Technologies, Inc.
CellVue is a registered trademark of Phanos Technologies
Composants de kit seuls
Réf. du produit
Description
- Diluent C 10 mL
- PKH67 Cell Linker in ethanol 0.1 mL
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Flam. Liq. 2
Code de la classe de stockage
3 - Flammable liquids
Point d'éclair (°F)
55.4 °F - closed cup
Point d'éclair (°C)
13 °C - closed cup
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
A microfluidic study of megakaryocytes membrane transport properties to water and dimethyl sulfoxide at suprazero and subzero temperatures
Tseng HY, et al.
Biopreservation and Biobanking, 9(4), 355-362 (2011)
Exosomal MALAT1 derived from oxidized low-density lipoprotein-treated endothelial cells promotes M2 macrophage polarization
Huang C, et al.
Molecular Medicine Reports, 18(1), 509-515 (2018)
Haiyang Xu et al.
Stem cell research & therapy, 10(1), 381-381 (2019-12-18)
Mesenchymal stem cells (MSCs) play a significant role in cancer initiation and metastasis, sometimes by releasing exosomes that mediate cell communication by delivering microRNAs (miRNAs). This study aimed to investigate the effects of exosomal miR-133b derived from MSCs on glioma
Yuan Yang et al.
Experimental and therapeutic medicine, 20(6), 257-257 (2020-11-18)
Mesenchymal stem cell (MSC) infusion has become a novel therapeutic strategy for complex autoimmune diseases; however, few detailed studies have been performed to investigate the benefit and mechanism of MSC treatment on systemic sclerosis (SSc). The present study aimed to
Simone Moertl et al.
International journal of molecular sciences, 21(7) (2020-04-02)
Normal tissue toxicity is a dose-limiting factor in radiation therapy. Therefore, a detailed understanding of the normal tissue response to radiation is necessary to predict the risk of normal tissue toxicity and to development strategies for tissue protection. One component
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique