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Principaux documents

PF0100

Sigma-Aldrich

Protease Fluorescent Detection Kit

high sensitivity assay

Synonyme(s) :

Protease Assay Kit

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.26

Niveau de qualité

Conditions d'expédition

dry ice

Température de stockage

−20°C

Application

Protease Fluorescent Detection Kit has been used:
  • in the detection of proteolytic activity using casein based protease assay
  • for testing the extracellular proteolytic activity of fungal strains
  • for quantifying mitochondrial protease activity
  • for measuring the protease production from soil solution

Adéquation

Detection limit: approx. 50 ng trypsin

Quantité

Supplied with sufficient reagents for up to 200 one mL assays.
Supplied with sufficient reagents for up to 200 one mL assays.

The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.

Remarque sur l'analyse

Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).

Composants de kit seuls

Réf. du produit
Description

  • Incubation buffer 25 mL

  • FITC-casein solution 5 mL

  • Assay buffer 200 mL

  • Protease standard (trypsin) 20 μg

  • FITC control 50 mg

  • Trichloroacetic acid (TCA) solution 30 mL

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Les clients ont également consulté

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Céline Adam et al.
PloS one, 7(5), e38138-e38138 (2012-06-14)
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Using the USA300, methicillin-resistant Staphylococcus aureus strain LAC, we previously examined the impact of regulatory mutations implicated in biofilm formation on protease production and virulence in a murine sepsis model. Here we examined the impact of these mutations in the

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