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P5400

Sigma-Aldrich

Pectinesterase from orange peel

greener alternative

lyophilized powder, ≥150 units/mg protein

Synonyme(s) :

Pectin methylesterase, Pectin pectylhydrolase

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About This Item

Numéro CAS:
9025-98-3
Numéro de classification (Commission des enzymes):
3.1.1.11 (BRENDA, IUBMB)
Numéro CE :
232-807-0
Numéro MDL:
MFCD00131812
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

lyophilized powder

Niveau de qualité

200

Activité spécifique

≥150 units/mg protein

Composition

Protein, 20-50% biuret

Caractéristiques du produit alternatif plus écologique

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Autre catégorie plus écologique

, Enabling

Température de stockage

2-8°C

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Catégories apparentées

Description générale

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Application

Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH4)2SO4 and sodium chloride. It has been used to study the pectin methylesterase gene.
The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis. It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.

Actions biochimiques/physiologiques

Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.

Définition de l'unité

One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.

Forme physique

Contains (NH4)2SO4 and sodium chloride

Remarque sur l'analyse

Protein determined by biuret.

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Alexis Peaucelle et al.
Current biology : CB, 18(24), 1943-1948 (2008-12-23)
Plant organs are produced from meristems in a characteristic pattern. This pattern, referred to as phyllotaxis, is thought to be generated by local gradients of an information molecule, auxin. Some studies propose a key role for the mechanical properties of
On-line characterization using ultrasound of pectin hydrolysis catalyzed by the enzyme pectinmethylesterase.
Cristina A, et al.
IOP Conference Series: Materials Science and Engineering, 42 (2012)
Sebastian Wolf et al.
Current biology : CB, 22(18), 1732-1737 (2012-08-14)
Brassinosteroid (BR) signaling is required for normal plant growth as shown by the dwarf phenotype of loss-of-function BR biosynthetic or perception mutants. Despite a detailed understanding of the BR signaling network, it is not clear how exactly BRs control growth.
Kirk E Francis et al.
Plant physiology, 142(3), 1004-1013 (2006-09-19)
Arabidopsis (Arabidopsis thaliana) QUARTET (QRT) genes are required for pollen separation during normal floral development. In qrt mutants, the four products of microsporogenesis remain fused and pollen grains are released as tetrads. In Arabidopsis, tetrad analysis in qrt mutants has
Ida Barbara Reca et al.
Plant molecular biology, 79(4-5), 429-442 (2012-05-23)
A pectin methylesterase inhibitor (SolyPMEI) from tomato has been identified and characterised by a functional genomics approach. SolyPMEI is a cell wall protein sharing high similarity with Actinidia deliciosa PMEI (AdPMEI), the best characterised inhibitor from kiwi. It typically affects
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