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Principaux documents

N9284

Sigma-Aldrich

Nitroreductase from Escherichia coli

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≥90% (SDS-PAGE), recombinant, expressed in E. coli

Synonyme(s) :

NTRA

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About This Item

Numéro MDL:
MFCD03457727
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Produit recombinant

expressed in E. coli

Niveau de qualité

200

Essai

≥90% (SDS-PAGE)

Forme

lyophilized powder

Activité spécifique

≥100 units/mL

Poids mol.

monomer 24000

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Numéro d'accès UniProt

P38489

Autre catégorie plus écologique

, Re-engineered

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

Escherichia coli K12 ... nfsB(945483)

Description générale

Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.

Application

Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.

Actions biochimiques/physiologiques

Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.
Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.
Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.
Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.

Propriétés physiques

Lyophilized powder containing PBS. Does not contain BSA as excipient

Définition de l'unité

One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.

Notes préparatoires

Produced using animal component-free materials.

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000206122
SLBZ7010
SLBR5273V
011M7677
109K3788

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Consulter la Bibliothèque de documents

Sébastien Jenni et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 248, 119179-119179 (2020-11-30)
The ever-growing demand for fluorogenic dyes usable in the rapid construction of analyte-responsive fluorescent probes, has recently contributed to a revival of interest in the chemistry of diketopyrrolopyrrole (DPP) pigments. In this context, we have explored the potential of symmetrical
Kinetics-Based Measurement of Hypoxia in Living Cells and Animals Using an Acetoxymethyl Ester Chemiluminescent Probe
Ryan LS, et al.
ACS sensors (2019)
Nitroreductase (GlNR1) increases susceptibility of Giardia lamblia and Escherichia coli to nitro drugs.
Nillius, D. J.
Antimicrobial Agents and Chemotherapy, 66, 1029-1035 (2011)
Structural and Mechanistic Studies of Escherichia coli Nitroreductase with the Antibiotic Nitrofurazone REVERSED BINDING ORIENTATIONS IN DIFFERENT REDOX STATES OF THE ENZYME
Race PR, et al.
The Journal of Biological Chemistry, 280(14), 13256-13264 (2005)
G M Anlezark et al.
Biochemical pharmacology, 50(5), 609-618 (1995-08-25)
A nitroreductase isolated and purified from Escherichia coli B has been demonstrated to have potential applications in ADEPT (antibody-directed enzyme prodrug therapy) by its ability in vitro to reduce dinitrobenzamides (e.g. 5-aziridinyl 2,4-dinitrobenzamide, CB 1954 and its bischloroethylamino analogue, SN
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