H8651

HEPES sodium salt

BioXtra, ≥99.0% (titration)

• Synonyme(s) : Acide 4-(2-hydroxyéthyl)pipérazine-1-éthanesulfonique sodium salt, Acide N-(2-hydroxyéthyl)pipérazine-N′-(2-éthanesulfonique) sodium salt
• Formule empirique (notation de Hill): C₈H₁₇N₂NaO₄S
• Numéro CAS: 75277-39-3
• Poids moléculaire : 260.29
• Numéro CE : 278-169-7
• Numéro MDL: MFCD00036463
• Code UNSPSC : 12161700
• ID de substance PubChem : 24895527
• Nomenclature NACRES : NA.25

Propriétés

• Gamme de produits: BioXtra
• Pureté: ≥99.0% (titration)
• Forme: powder
• Impuretés: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• pH: 10.0-12.0 (1 M in H₂O)
• Plage de pH utile: 6.8-8.2
• pKa (25 °C): 7.5
• Solubilité: H₂O: 1 M, clear, colorless
• Traces d'anions: chloride (Cl^-): ≤0.05%; sulfate (SO₄^2-): ≤0.05%
• Traces de cations: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Pb: ≤0.001%; Zn: ≤0.0005%
• Absorption: ≤0.2 at 260 in H₂O at 1 M; ≤0.2 at 280 in H₂O at 1 M
• Application(s): diagnostic assay manufacturing
• Chaîne SMILES: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• Clé InChI: RDZTWEVXRGYCFV-UHFFFAOYSA-M

Description

Description générale

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Application

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Autres remarques

Easily compare specifications for HEPES sodium salt products with the HEPES sodium salt specification table.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)