GE17-0963-03
Protein A Sepharose™ Cl-4B
Cytiva 17-0963-03, pack of 25 mL
Synonyme(s) :
Protein A Column, Sepharose 4B
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About This Item
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56
Produits recommandés
ligand
native protein A
Conditionnement
pack of 25 mL
Fabricant/nom de marque
Cytiva 17-0963-03
Conditions de stockage
(20% Ehtanol)
Matrice
4% cross-linked agarose
Taille des particules
45-165 μm
Diamètre moyen
90 μm
cleaning
2-10
working range
3-9
Capacité
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
Température de stockage
2-8°C
Description générale
Protein A Sepharose™ CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Caractéristiques et avantages
- Well documented for immunoglobulin purification and fractionation
- Strong binding to Fc region of IgG
- High capacity - 20 mg human IgG/mL.
- Suited for immunoprecipitation procedures
Stockage et stabilité
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Remarque sur l'analyse
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Informations légales
Sepharose is a trademark of Cytiva
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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