Accéder au contenu
Merck
Toutes les photos(1)

Principaux documents

P3391

Millipore

Protéine A-agarose

lyophilized powder

Synonyme(s) :

Protein A–Agarose

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Numéro MDL:
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Forme

lyophilized powder

Niveau de qualité

Conditionnement

glass bottle of 1 g
glass bottle of 1.5 g
glass bottle of 250 mg
glass bottle of 5 g

Conditions de stockage

do not freeze

Ampleur du marquage

3 mg per mL

Technique(s)

affinity chromatography: suitable

Couleur

white to gray

Matrice

Sepharose CL-4B

Activation de la matrice

cyanogen bromide

Fixation de matrice

amino

Espaceur de matrice

1 atom

Capacité

≥16 mg/mL binding capacity (human IgG)

Solubilité

water: 1 g/L at 20 °C

Adéquation

conforms to structure for swelling capacity (1g swells to 4 to 5 mL)

Température de stockage

2-8°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.

Application

Protein A-Sepharose was used:
  • for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.
  • in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.
  • as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.
  • in immunoprecipitation of [32P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.
Protein A-Sepharose is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Quantité

Swelling: 1 g swells to approx. 4 ml.

Forme physique

Supplied as lyophilized powder stabilized with lactose and dextran.

Informations légales

Sepharose is a trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

M Iezzi et al.
Molecular endocrinology (Baltimore, Md.), 13(2), 202-212 (1999-02-11)
Insulin-secreting cells express four GTPases of the Rab3 family. After separation of extracts of INS-1 cells on a sucrose density gradient, the bulk of the A, B, and C isoforms was recovered in the fractions enriched in insulin-containing secretory granules.
Tracie A Hennen-Bierwagen et al.
Plant physiology, 149(3), 1541-1559 (2009-01-27)
Starch biosynthetic enzymes from maize (Zea mays) and wheat (Triticum aestivum) amyloplasts exist in cell extracts in high molecular weight complexes; however, the nature of those assemblies remains to be defined. This study tested the interdependence of the maize enzymes
P Hornbeck et al.
Molecular and cellular biology, 9(9), 3727-3735 (1989-09-01)
The regulation and expression of protein kinase C (PKC) and phosphomyristin C (PMC) (a principal substrate of PKC which is the major myristylated protein in lymphocyte and glioma lines that express it) in murine B and T lymphocytes were investigated.
Frédéric Sorgeloos et al.
Journal of virology, 85(7), 3690-3694 (2011-01-14)
The L protein encoded by Theiler's murine encephalomyelitis virus (TMEV) is a unique example of a picornaviral protein encoded by an alternative open reading frame. This protein is an important determinant of TMEV persistence in the mouse central nervous system.
V Ossipow et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(17), 8219-8223 (1993-09-01)
The CCAAT/enhancer-binding protein (C/EBP) alpha is a leucine zipper protein that is preferentially expressed in certain cell types, such as adipocytes and hepatocytes. Here we show that C/EBP alpha mRNA is translated into two major proteins, C/EBP-42 and C/EBP-30, that

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique