E7885
Elastase from porcine pancreas
lyophilized powder, suitable for cell culture
Synonyme(s) :
Elastase from hog pancreas, Pancreatopeptidase E
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About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.75
Produits recommandés
Source biologique
Porcine
Niveau de qualité
Forme
lyophilized powder
Activité spécifique
≥4 units/mg protein
Poids mol.
25.9 kDa
Conditionnement
pkg of 1 μmol
Technique(s)
cell culture | mammalian: suitable
pH
8
Adéquation
suitable for
Activité étrangère
Trypsin ≤50 BAEE units/mg protein
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Description générale
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.
Application
Elastase from porcine pancreas is used for the following applications:
- Cell culture techniques
- ELISA techniques
- Used in assay for elastase inhibitory activity
Actions biochimiques/physiologiques
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.
Conditionnement
Package size based on protein content
Définition de l'unité
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
Application
Réf. du produit
Description
Tarif
Inhibiteur
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Chromatograms
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