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Key Documents

D4522

Sigma-Aldrich

Deoxyribonucleic acid from calf thymus

Type XV, Activated, lyophilized powder

Synonyme(s) :

Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

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About This Item

Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
41106310
eCl@ss :
32160414
Nomenclature NACRES :
NA.52

Qualité

for molecular biology

Niveau de qualité

Type

Type XV

Forme

lyophilized powder

Température de stockage

−20°C

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Description générale

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Caractéristiques et avantages

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Quantité

One mg DNA is approx. 20 A260 units.

Notes préparatoires

Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Brian D Holt et al.
Advanced healthcare materials, 5(23), 3056-3066 (2016-12-08)
Synthetic biomaterials are poised to transform medicine; however, current synthetic options have yet to ideally recapitulate the desirable properties of native tissue. Thus, the development of new synthetic biomaterials remains an active challenge. Due to its excellent properties, including electrical
Benjamin D Dickson et al.
Molecules (Basel, Switzerland), 24(8) (2019-04-24)
Poly(ADP-ribose)polymerase (PARP) inhibitors (PARPi) have recently been approved for the treatment of breast and ovarian tumors with defects in homologous recombination repair (HRR). Although it has been demonstrated that PARPi also sensitize HRR competent tumors to cytotoxic chemotherapies or radiotherapy
Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.
H V APOSHIAN et al.
The Journal of biological chemistry, 237, 519-525 (1962-02-01)
Claudia Gomes et al.
Life science alliance, 4(11) (2021-09-11)
High levels of autoimmune antibodies are observed in COVID-19 patients but their specific contribution to disease severity and clinical manifestations remains poorly understood. We performed a retrospective study of 115 COVID-19 hospitalized patients with different degrees of severity to analyze
Juan Antonio Aguilar-Pimentel et al.
Scientific reports, 10(1), 18334-18334 (2020-10-29)
Sex steroids, such as estrogens and androgens, are important regulators of the humoral immune response. Studies in female mice have demonstrated that alteration of circulating estrogen concentration regulates antibody-mediated immunity. As males have normally little endogenous estrogen, we hypothesized that

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