CS0780
β-N-Acetylglucosaminidase Assay Kit
sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
Synonyme(s) :
Beta-NAG Activity Assay Kit
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About This Item
Produits recommandés
Utilisation
sufficient for 50 reactions (1 mL)
sufficient for 500 reactions (100 μL)
Niveau de qualité
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Informations sur le gène
human ... NAGLU(4669)
Application
The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.
Actions biochimiques/physiologiques
N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.
Remarque sur l'analyse
The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.
Composants de kit seuls
Réf. du produit
Description
- Dilution Buffer 8 mL
- 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
- Citrate Buffer Solution, 0.09 M 100 mL
- p-Nitrophenol Standard Solution, 10 mM 1 mL
- β-N-Acetylglucosaminidase from Jack beans 1 vial
- Sodium carbonate 5 g
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral
Organes cibles
Liver,Kidney
Code de la classe de stockage
11 - Combustible Solids
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