GL0010
Golgi Isolation Kit
sufficient for 50 g (tissue)
Synonyme(s) :
Golgi Kit, Isolation Kit for Golgi
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32
Produits recommandés
Utilisation
sufficient for 50 g (tissue)
Niveau de qualité
Technique(s)
fractionation: suitable
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).
Application
Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.
Remarque sur l'analyse
The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mLFDS
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
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