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C3228

Sigma-Aldrich

CelLytic™ MT Cell Lysis Reagent

For mammalian tissues

Synonyme(s) :

Cell lysis buffer, Cell lysis reagent

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About This Item

Code UNSPSC :
41116134
Nomenclature NACRES :
NA.56

Application

CelLytic™ MT Cell Lysis Reagent has been used in the homogenization of hippocampi tissues, quadriceps muscles from mice, primary human trophoblasts (PHT), soleus muscles from mice and organs of Corti (OCs).

Caractéristiques et avantages

  • Gentle: Non-denaturing and does not interfere with downstream applications
  • Convenient: Provided ready to use

Autres remarques

CelLytic MT is an efficient reagent for the extraction of proteins. The lysis buffer consists of a dialyzable mild detergent, bicine, and 150 mM NaCl, resulting in minimal interference with protein interactions and biological activity. CelLytic MT is also used for extraction of cell-line proteins. A volume of 20 mL of CelLytic MT is sufficient for 1 gram of tissue.

Qualité

It has been tested, but not limited to the following tissues: rat brain, kidney, muscle, heart, liver, spleen and mouse brain, kidney and muscle.

Informations légales

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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de Lima EA, et al.
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Pasireotide protects mammalian cochlear hair cells from gentamicin ototoxicity by activating the PI3KAkt pathway
Kucharava K, et al.
Cell death & disease, 10(2), 110-110 (2019)
Mechanistic Target of Rapamycin Complex 1 Promotes the Expression of Genes Encoding Electron Transport Chain Proteins and Stimulates Oxidative Phosphorylation in Primary Human Trophoblast Cells by Regulating Mitochondrial Biogenesis
Rosario FJ, et al.
Scientific Reports, 9(1), 246-246 (2019)
Joseph S Marino et al.
The Journal of biological chemistry, 291(50), 25776-25788 (2016-10-28)
Glucocorticoids (GCs) regulate energy supply in response to stress by increasing hepatic gluconeogenesis during fasting. Long-term GC treatment induces hepatic steatosis and weight gain. GC signaling is coordinated via the GC receptor (GR) GRα, as the GRβ isoform lacks a
Terry D Hinds et al.
The Journal of biological chemistry, 291(48), 25179-25191 (2016-10-21)
Non-alcoholic fatty liver disease is the most rapidly growing form of liver disease and if left untreated can result in non-alcoholic steatohepatitis, ultimately resulting in liver cirrhosis and failure. Biliverdin reductase A (BVRA) is a multifunctioning protein primarily responsible for
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