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APOAC

Sigma-Aldrich

Annexin V-Cy3™ Apoptosis Detection Kit

Synonyme(s) :

Red fluorescent Annexin V apoptosis assay

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32

Utilisation

sufficient for 200 tests

Niveau de qualité

200

Conditionnement

pkg of 1 kit

Technique(s)

flow cytometry: suitable

Application(s)

cell analysis
detection

Méthode de détection

fluorometric

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Caractéristiques et avantages

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Principe

Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Informations légales

Cy3 is a trademark of Cytiva

Composants de kit seuls

Réf. du produit
Description
  • Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • C50416-Carboxyfluorescein diacetate 10 mgFDS

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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