A7005
β-Amylase from sweet potato
Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)
Synonyme(s) :
1,4-α-D-Glucan maltohydrolase
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About This Item
Produits recommandés
Source biologique
sweet potato
Niveau de qualité
Type
Type I-B
Forme
ammonium sulfate suspension
Activité spécifique
≥750 units/mg protein (E1%/280)
Score du produit alternatif plus écologique
old score: 57
new score: 1
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Caractéristiques du produit alternatif plus écologique
Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
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Concentration
≥7.0 mg/mL
Autre catégorie plus écologique
Température de stockage
2-8°C
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Description générale
β-Amylases belongs to the class of amylolytic enzymes.
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We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
Application
β -Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. β -Amylase, from Sigma, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides. β-amylase from sweet potato has been used to examine the utility of the enzyme in inhibiting and removing Staphylococcus aureus biofilms. The enzyme has also been used to prepare β-limit dextrin from waxy maize starch.
β-Amylase from sweet potato has been used:
- in the hydrolysis of starch and starch-pectin combinations
- for comparing amylases from different sources in Staphylococcus aureus SH1000 biofilm inhibition and reduction assay
- for selenium speciation in samples
Actions biochimiques/physiologiques
β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. β-amylase from sweet potato is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry.
β-Amylases plays a major role in examining enzymatic promiscuity among glycosidases.
Définition de l'unité
One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 4.8 at 20 °C.
Forme physique
Crystalline suspension in 2.3 M (NH4)2SO4
Autres remarques
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Certificats d'analyse (COA)
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Les clients ont également consulté
Mechanisms of enzymatic glycosyl transfer
Comprehensive Natural Products II (2010)
Effect of cross-linking on the resistance to enzymatic hydrolysis of waxy maize starch and low-methoxy pectin
Khondkar D, et al.
Food Hydrocolloids, 23(2), 387-393 (2009)
Xin Qi et al.
Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques, 14(1), 60-66 (2011-04-20)
β-Limit dextrin has been studied for many years as a means to investigate the internal structures of amylose and amylopectin. However its role as an excipient in the pharmaceutical industry has never been reported. This paper is the first one
C G Cheong et al.
Proteins, 21(2), 105-117 (1995-02-01)
Sweet potato beta-amylase is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry. Its subunit consists of 498 amino acid residues (Mr 55,880). It has been crystallized at room temperature using polyethylene glycol 1500 as precipitant.
Rezarta Reimann et al.
Physiologia plantarum, 114(1), 2-12 (2002-05-02)
In turions of Spirodela polyrhiza (L.) Schleiden, net degradation of storage starch is controlled by a special low fluence response of phytochrome requiring illumination for several days. This light effect has been used to study protein-starch interactions that occur prior
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