Please consider that the condition of the stock enzyme is different from the reaction in which it is used. You will be using a small amount of enzyme in a buffered reaction that is adjusted to the optimal pH by the reaction buffer itself.
A0810
Endoglycosidase H from Streptomyces plicatus
recombinant, expressed in E. coli, buffered aqueous solution
Synonyme(s) :
β-N-Acetylglucosaminidase H
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.32
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Produits recommandés
Produit recombinant
expressed in E. coli
Niveau de qualité
Conjugué
(N-linked)
Forme
buffered aqueous solution
Conditions d'expédition
wet ice
Température de stockage
2-8°C
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Description générale
Endoglycosidase H or endo-β-N-acetylglucosaminidase H is an glycohydrolase. It is produced by Streptomyces plicatus and other Streptomyces species.[1]
Actions biochimiques/physiologiques
Endoglycosidase H is involved in cleaving the N-linked glycans present between the two N-acetylglucosamine (GlcNAc) residues in the core of the glycan chain in high-mannose sugars.[2]
Définition de l'unité
One unit will release N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per min at 37 °C at pH 5.5.
Forme physique
Solution in 20 mM Tris HCl, pH 7.5, containing 50 mM NaCl, 1 mM EDTA
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Les clients ont également consulté
High-Level Expression of Endo-
Freeze H H and Kranz C
Current Protocols in Molecular Biology, 0 17(3) (2010)
High-Level Expression of Endo-
Wang F, et al.
Testing, 10(3) (2015)
Ingeborg Stals et al.
PloS one, 7(7), e40854-e40854 (2012-08-04)
Endo-N-acetyl-β-D-glucosaminidases (ENGases) hydrolyze the glycosidic linkage between the two N-acetylglucosamine units that make up the chitobiose core of N-glycans. The endo-N-acetyl-β-D-glucosaminidases classified into glycoside hydrolase family 18 are small, bacterial proteins with different substrate specificities. Recently two eukaryotic family 18
Yazen Jmeian et al.
Analytical chemistry, 84(20), 8790-8796 (2012-09-18)
A novel online enzyme reactor incorporating peptide-N-glycosidase F (PNGase F) on a monolithic polymer support has been developed to allow the rapid simultaneous release of both neutral and acidic N-linked glycans from glycoproteins. The PNGase F monolithic reactor was fabricated
Wei Zhang et al.
Talanta, 85(1), 499-505 (2011-06-08)
Endoglycosidase is a class of glycosidases that specifically cleaves the glycosidic bond between two proximal residues of GlcNAc in the pentasaccharide core of N-glycan, leaving the innermost GlcNAc still attached to its parent protein, which provides a different diagnostic maker
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Could you provide guidance on adjusting the pH of a solution that has a pH of 7.5, even though the workable pH range mentioned in the data sheet is 5.0–6.0? Specifically, which acid would be best to use for adjusting the pH before using the solution?
1 answer-
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