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Key Documents

45359

Sigma-Aldrich

Epoxy Embedding Medium kit

embedding resin for electron microscopy

Synonyme(s) :

Epon substitute embedding medium kit

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
12171500
Nomenclature NACRES :
NA.47

Niveau de qualité

pH

5-7

Densité

1.9 g/cm3

Application(s)

hematology
histology

Température de stockage

2-8°C

Description générale

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

Application

Epoxy Embedding Medium Kit has been used in the following applications:

  • to study the role of NDRG2, an essential colonic epithelial barrier regulator, in gut homeostasis maintenance and colitis-associated tumor development
  • to study the interaction of polymer scaffold nanofibers with growing cells
  • to study the morphological characterization of postembryonic development of blood–spleen barrier in ducks
  • to understand the role of NOD1/NOD2 receptors in Fusobacterium nucleatum-mediated neutrophil extracellular traps

Caractéristiques et avantages

  • Excellent medium for embedding a wide variety of tissues, including both plant and animal.
  • Facilitates rapid embedding (less than 3 hours).
  • Low viscosity facilitates rapid tissue penetration.
  • Desirable hardness is easy to obtain to suit a specific tissue type.

Composants

45345 Epoxy-Einbettungsmittel 2x250 ml
45347 Härter MNA 250 ml
45356 Härter DDSA 250 ml
45348 Beschleuniger DMP 30 250 ml

Informations légales

Epon is a trademark of Hexion, Inc.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Health hazardCorrosionExclamation mark

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Oral - Aquatic Chronic 4 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

275.0 °F

Point d'éclair (°C)

135 °C


Certificats d'analyse (COA)

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Les clients ont également consulté

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A Sawaguchi et al.
Journal of microscopy, 234(2), 113-117 (2009-04-29)
The goal of specimen preparation for transmission electron microscopy is to obtain high-quality ultra-thin sections with which we can correlate cellular structure to physiological function. In this study, we newly developed a capsule-supporting ring that can be useful for resin
Arnold Lavaud et al.
Translational vision science & technology, 9(5), 13-13 (2020-08-22)
To evaluate visual streak (VS) identification on spectral-domain optical coherence tomography (SD-OCT) scans in awake rabbits. To report thickness measurements in the VS and adjacent retina on OCT B-scans and histologic sections and to assess inter-method bias, precision and repeatability
High resolution 3D microscopy study of cardiomyocytes on polymer scaffold nanofibers reveals formation of unusual sheathed structure
Balashov, et al.
Acta Biomaterialia (2018)
Sevinç Kenan et al.
Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery, 47(3), 473-483 (2019-01-09)
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Role of NOD1/NOD2 receptors in Fusobacterium nucleatum mediated NETosis
Alyami, et al.
Microbial Pathogenesis (2019)

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