230914

Chymotrypsin Substrate II, Fluorogenic

Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity.

• Synonyme(s) : Chymotrypsin Substrate II, Fluorogenic, Suc-AAPF-AMC
• Formule empirique (notation de Hill): C₃₄H₃₉N₅O₉
• Numéro CAS: 88467-45-2
• Poids moléculaire : 661.70
• Numéro MDL: MFCD00080246
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Pureté: ≥98% (HPLC)
• Forme: lyophilized solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated; protect from light
• Couleur: white
• Solubilité: DMSO: soluble; acetonitrile: water (1:1): soluble
• Fluorescence: λ_ex ~380 nm; λ_em ~460 nm
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• InChI: 1S/C34H39N5O9/c1-19-16-30(43)48-27-18-23(11-12-24(19)27)37-25(17-22-8-5-4-6-9-22)32(45)38-33(46)26-10-7-15-39(26)34(47)21(3)36-31(44)20(2)35-28(40)13-14-29(41)42/h4-6,8-9,11-12,16,18,20-21,25-26,37H,7,10,13-15,17H2,1-3H3,(H,35,40)(H,36,44)(H,41,42)(H,38,45,46)/t20-,21-,25-,26-/m0/s1
• Clé InChI: IGKPQFYMWKQDQS-KCXKOMAXSA-N

Description

Description générale

Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity (excitation maximum: ~380 nm; emission maximum: ~460 nm).

Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity.

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
Chymotrypsin

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Standard Handling (A)

Séquence

Suc-Ala-Ala-Pro-Phe-AMC

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Irvine, G.B., et al. 1990. Anal. Biochem.185, 304.
Graf, L., et al. 1987. Biochemistry26, 2616.
Oshima, G. 1984. J. Biochem.95, 1131.
Oshima, G. 1983. J. Biochem.94, 1615.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable