17-500M
Catch and Release v2.0 Reversible Immunoprecipitation System
Synonyme(s) :
Catch and release system, Immunoprecipitation kit
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About This Item
Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NB.22
Produits recommandés
Réactivité de l'espèce (prédite par homologie)
all
Niveau de qualité
Fabricant/nom de marque
Catch And Release
Upstate®
Technique(s)
activity assay: suitable (IP-kinase)
immunoprecipitation (IP): suitable
western blot: suitable
Conditions d'expédition
wet ice
Description générale
Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
Application
Catch and Release v2.0 should not be used with proteins containing a 6-His tag.
Conditionnement
50 immunoprecipitations
Composants
Catch and Release Wash Buffer, 10X (Cat.# 20-210)
Catch and Release Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
Catch and Release Denaturing Elution Buffer, 1X (Cat.# 20-284)
20-285
20-216
Catch and Release Capture Tubes
Catch and Release Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
Catch and Release Denaturing Elution Buffer, 1X (Cat.# 20-284)
20-285
20-216
Catch and Release Capture Tubes
Qualité
routinely evaluated by immunoprecipitation.
Stockage et stabilité
All components to be stored at 4°C except the Catch and Release Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
Code de la classe de stockage
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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