14-770-M
mTOR (1362-end) Protein, active, 10 µg
Active, N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end, for use in Kinase Assays.
Synonyme(s) :
FRAP1
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About This Item
Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in baculovirus infected Sf21 cells
Forme
liquid
Activité spécifique
377 U/mg
Fabricant/nom de marque
Upstate®
Technique(s)
activity assay: suitable (kinase)
Solubilité
water: soluble
Numéro d'accès NCBI
Numéro d'accès UniProt
Température de stockage
−70°C
Informations sur le gène
human ... MTOR(2475)
Description générale
Research area: Cell Signaling
Protein Target: mTOR (FRAP1)
Target Sub-Family: Lipid/Atypical
N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end
The mechanistic target of rapamycin (mTOR) protein is a serine/threonine protein kinase belonging to PI3K-related kinase (PIKK) family, expressed throughout the body.
Protein Target: mTOR (FRAP1)
Target Sub-Family: Lipid/Atypical
N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end
The mechanistic target of rapamycin (mTOR) protein is a serine/threonine protein kinase belonging to PI3K-related kinase (PIKK) family, expressed throughout the body.
Application
The active mTOR (1362-end) protein, 10 µg, has been used in the mTOR substrate phosphorylation assay to validate the biochemical and pharmacological profile of a novel mTOR-KI for targeting mTOR signaling pathways in vitro and in vivo.
Actions biochimiques/physiologiques
Mechanistic target of rapamycin (mTOR) plays a pivotal role in regulating various essential cellular processes, ranging from protein synthesis to autophagy. Moreover, it influences metabolism, cellular survival, gene transcription, and cytoskeletal components. It gets activated in response to growth factors, nutrients, mitogens, and hormones.Downregulation of mTOR signaling is associated with the advancement of cancer and diabetes, and aging process.
Conditionnement
Also available in 100μg size, please inquire for pricing and availability.
Définition de l'unité
Specific Activity: where one unit of mTOR activity is defined as 1 nmole of phosphate incorporated into 2mg/ml mTOR substrate per minute at 30°C with a final ATP concentration of 100µM.
Forme physique
16µg of enzyme in 100µl of 10mM HEPES, 50mM NaCl, 50mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 300µg/ml FLAGTM peptide, 20% v/v glycerol
Stockage et stabilité
Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
mTOR Signaling in Growth, Metabolism, and Disease
Saxton RA and Sabatini DM
Cells, 168(6), 960?976- 960?976 (2017)
mTOR: Exploring a New Potential Therapeutic Target for Stroke
Castellanos M, et al.
Molecules to Medicine with mTOR, 105-122 (2016)
Jianchang Qian et al.
Oncotarget, 7(41), 67071-67086 (2016-08-27)
The mechanistic target of rapamycin (mTOR) is a rational target for cancer treatment. While the mTORC1-selective rapalogs have shown significant benefits in the clinic, antitumor response may be further improved by inhibiting both mTORC1 and mTORC2. Herein, we established target
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