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A9226

Sigma-Aldrich

Alkaline buffer solution

1.5 M, pH 10.3 (25 °C)

Synonyme(s) :

Basic pH buffer

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About This Item

Code UNSPSC :
12161700
Nomenclature NACRES :
NA.25

Forme

liquid

Niveau de qualité

Concentration

1.5 M

pH

10.3 (25 °C)

Température de stockage

2-8°C

Description générale

Alkaline buffer solution, 1.5 M, pH 10.3 at 25 °C is used in alkaline phosphatase reactions. It contains 2-amino-2-methylpropanol (AMP) and is used in conjunction with p-nitrophenyl phosphate (pNPP) enzyme substrate.

Application

Alkaline buffer solution has been used to measure the alkaline phosphatase (ALP) activity in the:
  • rat bone marrow stromal cells (BMSC)
  • osteoblast-like cells (SaoS-2, ATCC HTB 85)
  • mouse MC3T3-E1

Autres remarques

Contains 2-amino-2-methylpropanol

Pictogrammes

Corrosion

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Dam. 1 - Skin Irrit. 2

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Young-Hee Kim et al.
BMC pharmacology & toxicology, 14, 60-60 (2013-12-03)
Indoxyl sulfate (IS), an organic anion uremic toxin, promotes the progression of renal dysfunction. Some studies have suggested that IS inhibits osteoclast differentiation and suppresses parathyroid hormone (PTH)-stimulated intracellular cAMP production, decreases PTH receptor expression, and induces oxidative stress in
Pin Huang et al.
Colloids and surfaces. B, Biointerfaces, 160, 628-638 (2017-10-17)
Increased use of reconstruction procedures in orthopedics has improved the life of patients undergoing surgery. However, surgical site infection remains a major challenge. Efforts were made to fabricate antibacterial surfaces with good biocompatibility. This present study aimed to fabricate zinc-incorporated
Salima Nedjari et al.
Scientific reports, 7(1), 15947-15947 (2017-11-23)
Stem cells therapy offers a viable alternative for treatment of bone disorders to the conventional bone grafting. However clinical therapies are still hindered by the insufficient knowledge on the conditions that maximize stem cells differentiation. Hereby, we introduce a novel
C Caparrós et al.
Journal of the mechanical behavior of biomedical materials, 39, 79-86 (2014-08-12)
The generation of titanium foams is a promising strategy for modifying the mechanical properties of intervertebral reinforcements. Thus, the aim of this study was to compare the in vitro biological response of Ti6Al4V alloys with different pore sizes for use
Helle Lysdahl et al.
BioResearch open access, 3(6), 278-285 (2014-12-04)
Clinical trials using bone morphogenetic protein-2 (BMP2) for bone reconstruction have shown promising results. However, the relatively high concentration needed to be effective raises concerns for efficacy and safety. The aim of this study was to investigate the osteogenic effect

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