796-0150

HPLC Analysis of Acidic Herbicides in Water on a Polymeric C18 Column after SPE using Supelclean^™ ENVI^™-Carb

application for SPE, application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable; solid phase extraction (SPE): suitable
• Testparameter: sample preparation: SPE (Solid Phase Extraction); sample/matrix: Fresh 1 L water samples, dechlorinated with sodium thiosulfate when necessary, at ambient temperature and pH.; SPE tube/cartridge: Supelclean ENVI-Carb, 250 mg/6 mL (57092); condition: 10 mL DI water at 20 mL/min; sample addition: 0.9 L water sample at 20 mL/min; drying: 10 min using clean nitrogen; washing: 10 mL DI water at 20 mL/min; elution: 10 mL 0.1% phosphoric acid in methylene chloride:acetonitrile (80:20) at 5 mL/min; column: polymeric-coated silica-based PAH specialty column, 20 cm × 3 mm I.D., 5 μm (Supelco equivalent, SUPELCOSIL LC-PAH, available upon request); mobile phase: gradient, [A]: 0.05% phosphoric acid in DI water; [B]: acetonitrile; flow rate: 0.5 mL/min; column temp.: 50 °C; detector: photodiode array- peak width: 0.053 min, sampling interval: 0.320 sec, monitor 210 nm & 225 nm; injection: 10 μL of extract (4-5 ppb each analyte in water)
• Eignung: application for HPLC; application for SPE
• Anwendung(en): environmental

Beschreibung

Anwendung

using Zymark AutoTrace Extraction WorkStation 1.20

Rechtliche Hinweise

ENVI is a trademark of Sigma-Aldrich Co. LLC

Supelclean is a trademark of Sigma-Aldrich Co. LLC

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