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T3824

Sigma-Aldrich

Tyrosinase aus Ständerpilz

Tyrosinase from mushroom

lyophilized powder, ≥1000 unit/mg solid

Synonym(e):

Katechol-Oxidase, Monophenol, Dihydroxyphenylalanin: Sauerstoff-Oxidoreduktase, Monophenol-Monooxygenase, Polyphenol-Oxidase

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About This Item

CAS-Nummer:
9002-10-2
EC-Nummer:
1.14.18.1 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00082118
UNSPSC-Code:
12352204
NACRES:
NA.54
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Biologische Quelle

fungus (mushroom)

Qualitätsniveau

200

Form

lyophilized powder

Spezifische Aktivität

≥1000 unit/mg solid

Mol-Gew.

119.5 kDa by electrophoresis
 128 kDa by sedimentation velocity diffusion
 133 kDa by LS

Anwendung(en)

diagnostic assay manufacturing

Versandbedingung

wet ice

Lagertemp.

−20°C

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Verwandte Kategorien

Allgemeine Beschreibung

Isoelektrischer Punkt (pl): 4,7 - 5
Der optimale pH ist 6 - 7

Molekulargewicht: 128 kDa mithilfe von Diffusion der Sedimentationsgeschwindigkeit; 133 kDa mithilfe von Lichtstreuungsmessungen und 119,5 kDa mithilfe von Elektrophorese.

Tyrosinase ist eine kupferhaltige Oxidase, die auf Catechine und Cresol reagiert. Sie ist für Bräunungsvorgänge verantwortlich. Berichten zufolge hat das Enzym zwei Bindungsstellen für aromatische Substrate und eine weitere Bindungsstelle für Sauerstoff-Kupfer. Das Kupfer ist wahrscheinlich Cu(I), bei Inaktivierung mit Oxidation zu Cu(II)-Ion.
Tyrosinase ist eine kupferhaltige Oxidase, die auf Catechine und Cresol reagiert. Tyrosinase nimmt an der Umwandlung von Tyrosin in Dihydroxyphenylalanin (DOPA) teil. Die Polymerisation von DOPA führt zur Bildung von Melanin. Tyrosinase spielt eine Rolle in Bräunungsreaktionen. Das Enzym verfügt offenbar über zwei Bindungsstellen für aromatische Substrate und eine eindeutige Bindungsstelle für Sauerstoff-Kupfer.

Anwendung

Tyrosinaseaktivität wurde in einer Studie zur Entwicklung eines alternativen Therapeutikums zur Behandlung der Hyperpigmentation untersucht. [1] Tyrosinase wurde auch in einer Studie eingesetzt, die den okulokutanen Albinismus-Phänotyp in der pakistanischen Bevölkerung untersuchte. [2]

Biochem./physiol. Wirkung

Wandelt Tyrosin in L-DOPA oder Tyramin in Dopamin um

Physikalische Eigenschaften

Isoelektrischer Punkt (pl): 4,7 - 5
pH (6 - 7 ist optimal)

Molekulargewicht: 128 kDa durch Sedimentationsgeschwindigkeitsdiffusion, 133 kDa durch Lichtstreuungsmessungen und 119,5 kDa durch Elektrophorese.

Einheitendefinition

Eine Einheit = ΔA280 von 0,001 pro min bei pH 6,5 und 25 °C in 3-ml-Reaktionsgemisch mit L-Tyrosin.

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000428964
0000428963
0000428963
0000428964
0000428961

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

Suche nach COA

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L-Tyrosin from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 99.0-101.0%

Sigma-Aldrich

T8566

L-Tyrosin

N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala

Sigma-Aldrich

F5135

N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala

Anti-Tyrosinase-Antikörper, Klon T311 clone T311, Upstate®, from mouse

Sigma-Aldrich

05-647

Anti-Tyrosinase-Antikörper, Klon T311

A T S Jorge et al.
International journal of cosmetic science, 34(5), 435-440 (2012-06-29)
Several treatments for skin whitening are available today, but few of them are completely adequate, especially owing to the carcinogenic potential attributed to classical drugs like hydroquinone, arbutin and kojic acid. To provide an alternative and safer technology for whitening
Shilpi Goenka et al.
Molecules (Basel, Switzerland), 25(16) (2020-08-13)
Previous studies have reported that estrogen hormone promotes melanogenesis while progesterone inhibits it. A selective estrogen receptor modulator (SERM), tamoxifen, has been shown to promote melanogenesis; however, to date, there have been no reports on the effects of a selective
Rongchao Zhang et al.
Evidence-based complementary and alternative medicine : eCAM, 2020, 2582579-2582579 (2020-08-11)
Whitening cosmetics market has a bright future, and pure natural whitening products of traditional Chinese medicine have always been a research hotspot. In this research, the whitening active ingredient of Chinese medicine Trichosanthes pulp was isolated and purified for the
Tyrosinase inhibitory effects and inhibition mechanisms of nobiletin and hesperidin from citrus peel crude extracts.
Zhang, et al.
Journal of Enzyme Inhibition and Medicinal Chemistry, 22, 83-90 (2019)
Thomas J Jaworek et al.
Orphanet journal of rare diseases, 7, 44-44 (2012-06-28)
Oculocutaneous albinism (OCA) is caused by a group of genetically heterogeneous inherited defects that result in the loss of pigmentation in the eyes, skin and hair. Mutations in the TYR, OCA2, TYRP1 and SLC45A2 genes have been shown to cause
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2 Reviews This action will navigate to reviews.

Questions

1–10 of 10 Questions  

  1. When measuring the activity of Tyrosinase, should we use the highest change observed every minute between Tyrosinase and the mixture (with L-tyrosine) as the activity, or the average change over the measured time?

    1 answer

    1. Refer to the protocol of the enzymatic assay of Tyrosinase as described in the reference: Duckworth, H. W. and Coleman, J. E. (1970) Journal of Biological Chemistry, 245, 1613-1625. The protocol can be accessed at https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/776/246/tyrosinase.pdf

      Helpful?

  2. Is this tyrosinase is derived from Agaricus bisporus or another mushroom species?

    1 answer

    1. Yes, this product is derived from Agaricus bisporus.

      Helpful?

  3. Can you please tell me 25000 units tyrosinase in milligrams?

    1 answer

    1. This product is sold by activity. The minimum activity specification for this material is 1000 units per milligram. The weight of this product can be determined using the activity value reported on the lot specific Certificate of Analysis(COA). For example, the sample COA has a activity value of 6540 units/mg. The amount of powder in the 25,000 unit package will be 3.82mg.

      Please see the link below or navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/US/en/product/sigma/t3824#product-documentation

      Helpful?

  4. Hi, How much is the total protein amount of T3824 (25KU) per vial?

    1 answer

    1. The protein content of this product is not determined. This product is tested and released based on the activity per milligram solid. Should the protein content be required, please see the link below to review several kits that may be of interest:
      https://www.sigmaaldrich.com/US/en/product/compare?term=total%20protein%20kit&facet%5B0%5D=facet_product_category%3Akits&focus=products&page=1&perpage=30&sort=relevance&type=product&products=TP0300%3ASIGMA%3Asial%2CTP0200%3ASIGMA%3Asial%2CTP0100%3ASIGMA%3Asial

      Helpful?

  5. What solution is best to be used to reconstitute the T3824-25KU? and what is the volume and concetration of that soluation?

    1 answer

    1. Tyrosinase is soluble at 2 mg/mL in 50 mM potassium phosphate buffer. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/377/683/t3824dat-ms.pdf

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. How can Tyrosinase from mushroom, Product T3824, be dissolved and what is its expected stability in solution?

    1 answer

    1. Tyrosinase can be dissolved in 50 mM potassium phosphate buffer at 2 mg/mL.  Solutions are expected to retain activity for several days in the refrigerator (2-8°C) and for several weeks in the freezer (-20°C).

      Helpful?

  8. I see that the approximate activity of Product T3824, Tyrosinase from mushroom, is  ?1000 unit/mg solid, but how can I find the lot specific activity of this tyrosinase?

    1 answer

    1. The certificate of analysis for the lot will report the exact tyrosinase activity for a particular batch. The lot number can be found on the label of the bottle, or you can call technical service (800-325-5832) for a sample lot number to view the online certificate of analysis.

      Helpful?

  9. The website says Product T3824, Tyrosinase from mushroom, should be stored at -20°C, but I received it at room temperature. Is this correct?

    1 answer

    1. The product is shipped at ambient temperature and is stable for up to two weeks during shipment.  Once received, it is recommended to store the product at -20°C to insure the quality for long term storage.

      Helpful?

  10. I used to order another tyrosinase (Product No. T7755), which has since been discontinued. What is the difference between the replacement product T3824 and the discontinued product T7755?

    1 answer

    1. The difference between these two products is that T3824 has been stabilized with trehalose, while T7755 was not.

      Helpful?

Reviews

Active Filters

  1. Madrid, Spain
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Tyrosinase from mushroom

    All the products arrived in due time and storage conditions.

    Helpful?

  2. 1 Ratings-Only Review

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