STA20

Stärken (GO/P)-Assay-Kit

sufficient for 20 assays

• Synonym(e): Starch Measurement Kit
• NACRES: NA.84
• UNSPSC Code: 12164500

Eigenschaften

• usage: sufficient for 20 assays
• Quality Level: 100
• application(s): food and beverages; general analytical
• storage temp.: 2-8°C

Beschreibung

General description

Starch (GO/P) Assay Kit was used to determine the starch content, during an experimental study done in order to investigate the composition and structure of sorghum spent grains (SSG), which is a brewery co-product and is reported to have potential applications thereby adding value to the product. It was also used in starch extraction and quantification, during investigating the importance of OsGA2ox5 in GAs homeostasis, development, gravity responses and stress tolerance in rice. Furthermore, it also found application in being used in analysing factors affecting polyhydroxybutyrate accumulation , which are basically linear biodegradable polyesters produced by bacteria as a carbon store, in mesophyll cells of sugarcane and switchgrass.

Application

Dieses Kit ist vorgesehen für die quantitative, enzymatische Bestimmung von natürlicher Stärke in Nahrungsmmitteln und anderen Materialien. Die Hydrolyse von Stärke zu Glucose wird durch die α-Amylase und die Amyloglucosidase katalysiert Glucose wird durch Glucose-Oxidase zu Gluconsäure und Wasserstoffperoxid oxidiert. Wasserstoffperoxid reagiert mit o-Dianisidin in Gegenwart von Peroxidase zu einem gefärbten Produkt. Oxidiertes o-Dianisidin reagiert mit Schwefelsäure zu einem stabileren gefärbten Produkt. Die Intensität der rosa Färbung, gemessen bei 540 nm ist proportional zu der anfänglichen Glucosekonzentration.

Suitable for the quantitative, enzymatic determination of starch infood and other materials.

Starch (GO/P) Assay Kit was used to determine the starch content, during an experimental study done in order to investigate the composition and structure of sorghum spent grains (SSG), which is a brewery co-product and is reported to have potential applications thereby adding value to the product. It was also used in starch extraction and quantification, during investigating the importance of OsGA2ox5 in GAs homeostasis, development, gravity responses and stress tolerance in rice. Furthermore, it also found application in being used in analysing factors affecting polyhydroxybutyrate accumulation , which are basically linear biodegradable polyesters produced by bacteria as a carbon store, in mesophyll cells of sugarcane and switchgrass.

Biochem/physiol Actions

This kit is for the quantitative, enzymatic determination of starch in food and other materials. The hydrolysis of starch to glucose is catalyzed by α-amylase and amyloglucosidase. Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product. The intensity of the pink color measured at 540 nm is proportional to the original glucose concentration.

Nur Kit-Komponenten

• α-Amylase, Heat Stable .5 mL
• Starch Assay Reagent 1 mL/vial
• Glucose Oxidase/Peroxidase Reagent 1 capsule
• o-Dianisidine Reagent 5 mg
• Glucose Standard Solution .5 mL
• Wheat Starch 1 g
• Corn Starch 1 g

Sicherheitshinweise

• pictograms: GHS08,GHS05,GHS07
• signalword: Danger
• hcodes: H302,H314,H334,H335,H350
• pcodes: P202 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• wgk: WGK 3