SRP6014

HGF human

recombinant, expressed in CHO cells, ≥97% (SDS-PAGE), ≥97% (HPLC)

• Synonym(e): F-TCF, HGF, HGFB, Hepatopoietin (HPTA), Scatter Factor (SF)
• CAS-Nummer: 953836-17-4
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in CHO cells
• Assay: ≥97% (HPLC); ≥97% (SDS-PAGE)
• Form: lyophilized
• Mol-Gew.: 80.017 kDa
• Verpackung: pkg of 1 mg; pkg of 10 μg
• Methode(n): cell culture | embryo: suitable
• Verunreinigungen: Endotoxin, tested
• NCBI-Hinterlegungsnummer: P14210
• UniProt-Hinterlegungsnummer: P14210
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... HGF(3082)

Beschreibung

Allgemeine Beschreibung

Hepatocyte growth factor (HGF) is a multifunctional growth factor. HGF Human Recombinant produced in Chinese hamster ovary (CHO) is a heterodimer, non-glycosylated, polypeptide chain consisting of an α-chain of 463 amino acids and β-chain of 234 having a total molecular mass of 80.017kDa. The gene encoding this protein is localized on human chromosome 7q21.1. The HGF is purified by proprietary chromatographic techniques.

Biochem./physiol. Wirkung

Hepatocyte growth factor (HGF) regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with interleukin-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. It is expressed in brain tumors.

Physikalische Form

The protein was lyophilized from a concentrated (1 mg/ml) solution containing PBS pH 7.4 containing 0.05% Tween 20 and 100 mM L-arginine.

Rekonstituierung

Centrifuge the vial prior to opening. It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor (HGF) in sterile 18 MΩcm^-1 H₂O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable