SAB4200780

Anti-Human IgM (μ-chain specific) antibody, Mouse monoclonal

clone MB-11, purified from hybridoma cell culture

• Synonym(e): Anti-Human immunoglobulin M
• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 300
• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Klon: MB-11, monoclonal
• Form: buffered aqueous solution
• Speziesreaktivität: human
• Konzentration: ~1.0 mg/mL
• Methode(n): ELISA: 0.1-0.2 μg/mL using 2.5 μg/mL Human IgM myeloma for coating; immunoblotting: suitable
• Isotyp: IgG2b
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the MB-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgM protein.

Immunoglobulin M (IgM) is the major class of surface immunoglobulins on lymphocyte membranes. IgM is suggested to act as the first line of defense during microbial infections. IgM has a pentameric structure, in which monomers are linking together via disulfide bonds. Surface IgM is expressed on immature and mature B cells, whereas IgM heavy chain is expressed intracellularly in pre-B cells. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation.

Spezifität

Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal specifically recognizes m chain of Human IgM. The antibody shows no cross-reactivity with human IgG, Fab, Fc, k, l, and g chains.

Immunogen

Purified human IgM

Anwendung

The antibody is recommended to use in various immunological techniques, including ELISA and Immunoblot.

Biochem./physiol. Wirkung

Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Sonstige Hinweise

This product is for R&D use only, not for drug, household, or other uses.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable