SAB4200741

Anti-Human IgE antibody, Mouse monoclonal

clone GE-1, purified from hybridoma cell culture

• Synonym(e): Anti-Immunoglubulin E
• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Klon: GE-1, monoclonal
• Form: buffered aqueous solution
• Speziesreaktivität: human
• Konzentration: ~1.0 mg/mL
• Methode(n): ELISA: 0.15-0.3 μg/mL using 1 μg/mL Human IgE myeloma for coating; immunoblotting: suitable; immunoprecipitation (IP): 1-2 μg/test using Human IgE myeloma
• Isotyp: IgG2b
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Anti-Human IgE antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgE myeloma protein. Immunoglobulin E (IgE) is the least abundant immunoglobulin isotype in circulation. IgE is produced by plasma cells and is highly functional in the lung, skin and mucus membranes. Normal levels of IgE in serum are less than 1 μg/ml.

Immunogen

purified human IgE myeloma protein

Anwendung

Anti-Human IgE antibody, Mouse monoclonal has been used in various immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunoblot, immunoprecipitation and IgE quantification using piezoelectric (Pz) crystal bioassay.

Biochem./physiol. Wirkung

Immunoglobulin E (IgE) plays a critical role in allergic reactions and host protection from parasites. Serum IgE levels are highly elevated during an allergic reaction (such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease or primary immunodeficiency). Detection and measurement of total IgE concentrations is crucial for diagnosis, monitoring and treatment of allergy patients. In addition, the recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable