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SAB2702243

Sigma-Aldrich

Monoclonal Anti-5-Methylcytosine / 5-mC antibody produced in mouse

clone GT4111, affinity isolated antibody

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

GT4111, monoclonal

Form

buffered aqueous solution

Konzentration

1mg/mL

Methode(n)

dot blot: suitable
immunohistochemistry: suitable
methylated DNA immunoprecipitation (MeDIP): suitable

Isotyp

IgG1

Versandbedingung

wet ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Immunogen

The immunogen used to generate this antibody corresponds to 5-Methylcytosine / 5-mC

Anwendung

Suggested starting dilutions are as follows: Dot: Assay-dependent dilution, EM: Assay-dependent dilution, IHC-P: 1:100-1:1000, IHC-Wm: 1:50-1:500, MeDIP: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Leistungsmerkmale und Vorteile

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Sonstige Hinweise

Purification: Affinity purified by Protein G

Physikalische Form

Phosphate-buffered saline, no preservative added.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Cen Jiang et al.
Cell death discovery, 8(1), 46-46 (2022-02-04)
The morbidity of papillary thyroid cancer (PTC) is on the rise, but its pathogenesis is still poorly understood. NR4A1 is a transcription factor primarily involving a wide range of pathophysiological responses, but its relationship with PTC malignancy remains unclear. This
Gerhard P Aigner et al.
Toxics, 10(2) (2022-02-25)
Earthworms are well-established model organisms for testing the effects of heavy metal pollution. How DNA methylation affects cadmium (Cd) detoxification processes such as the expression of metallothionein 2 (MT2), however, is largely unknown. We therefore exposed Lumbricus terrestris to 200
Sunwang Xu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11(16), e2308531-e2308531 (2024-02-21)
Gallbladder cancer (GBC) is an extremely lethal malignancy with aggressive behaviors, including liver or distant metastasis; however, the underlying mechanisms driving the metastasis of GBC remain poorly understood. In this study, it is found that DNA methyltransferase DNMT3A is highly
Manthan Patel et al.
BMC genetics, 21(1), 84-84 (2020-07-31)
The human CGGBP1 binds to GC-rich regions and interspersed repeats, maintains homeostasis of stochastic cytosine methylation and determines DNA-binding of CTCF. Interdependence between regulation of cytosine methylation and CTCF occupancy by CGGBP1 remains unknown. By analyzing methylated DNA-sequencing data obtained
Sunwang Xu et al.
Journal of experimental & clinical cancer research : CR, 40(1), 373-373 (2021-11-27)
Gallbladder cancer (GBC) is known for its high malignancy and multidrug resistance. Previously, we uncovered that impaired integrity and stability of the elongator complex leads to GBC chemotherapy resistance, but whether its restoration can be an efficient therapeutic strategy for
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