The detergents and chaotropic agents in the kit will at least partially denature the proteins, making them unsuitable for use in co-immunoprecipitation assays.
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PE0230
Plant Total Protein Extraction Kit
Suitable for any plant species or tissue
Synonym(e):
Total Protein Extraction Kit
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|
Über diesen Artikel
NACRES:
NA.56
UNSPSC Code:
41105500
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenGeneral description
The Plant Total Protein Extraction Kit is designed specifically for use in plant bioscience to extract a qualitative sample of all proteins from any type of plant species or tissue. The protocol does not require any ultracentrifugation or aqueous polymer two-phase partitioning (APTP). The kit includes two reagents; a plant specific protease inhibitor cocktail and a new chaotropic reagent with increased solubilizing power to extract more hydrophobic proteins (C0356, Protein Extraction Reagent Type 4). Routine use of the reagent in sequential protein extraction of a plant tissue sample with fresh reagent removes nearly all protein from the tissue. The protease inhibitor cocktail is a mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, metalloproteases, aspartic, and aminopeptidases. It contains pepstatin A, 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), E-64, leupeptin, 1,10-phenanthroline, and bestatin. The cocktail has been demonstrated to be highly effective in preventing protein degradation during the extraction process.
Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in the chaotropic reagent. Plant debris is pelleted by centrifugation and the protein extract is collected. The end result is a qualitative total protein sample, ready for downstream proteomic analysis.
Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in the chaotropic reagent. Plant debris is pelleted by centrifugation and the protein extract is collected. The end result is a qualitative total protein sample, ready for downstream proteomic analysis.
Application
The plant total Protein Extraction Kit has been used to isolate crude seed protein. It has also been used to extract the total protein.
Preparation Note
Sufficient for preparation of 20 separate extractions from plant tissue samples of 10-250mg
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| technique(s) protein extraction: suitable | technique(s) - | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
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Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2
Lagerklasse
11 - Combustible Solids
flash_point_f
185.0 °F - closed cup
flash_point_c
85 °C - closed cup
wgk
WGK 3
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Verwandter Inhalt
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| PE0230-1KT | 04061834406853 |
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Hi there, I just wanted to find out if the samples extracted from this kit can be used in Co-IP or not?
1 answer-
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How can I improve my yield when using Product PE0230, Plant Protein Extraction Kit?
1 answer-
A second extraction can be performed and combined with the first to increase protein extracted to about 95% of the total.
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Will my protein samples be denatured when using Product PE0230, Plant Protein Extraction Kit?
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Yes. The chaotropic agents and the detergent in the kit will denature the proteins at least partially.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the expected protein yield when using Product PE0230, Plant Protein Extraction Kit?
1 answer-
Depending on the tissue used (age, type (leaf, stem, root) health, etc.) one can expect 1-4 mg/mL at about 75-80% of the total protein.
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