P7937

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit

5,000-15,000 units/mg protein, recombinant, expressed in E. coli, lyophilized powder

• Synonym(e): Protein phosphatase
• EC-Nummer: 3.1.3.16 (BRENDA, IUBMB)
• MDL-Nummer: MFCD01635658
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Assay: ≥80% (GE)
• Form: lyophilized powder
• Spezifische Aktivität: 5,000-15,000 units/mg protein
• Mol-Gew.: 37.5 kDa
• Methode(n): inhibition assay: suitable
• UniProt-Hinterlegungsnummer: P62139
• Lagertemp.: −20°C
• Angaben zum Gen: rabbit ... PPP1CA(100009298)

Beschreibung

Allgemeine Beschreibung

Protein phosphatase1 (PP1) is a heterodimeric enzyme with serine/threonine phosphatase activity. It comprises a catalytic subunit and a targeting subunit or a specific protein inhibitor. The targeting subunit determines the enzyme localization, e.g., to glycogen particles (subunit G) or to myofibrils (subunit M).

Anwendung

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used as a standard in protein phosphatase-1 inhibition assay.

Protein Phosphatase-1 Catalytic Subunit, α-Isoform from rabbit has been used in protein phosphatase reaction and in the digestion of adipocyte extracts.

Biochem./physiol. Wirkung

Protein phosphatase1 (PP1) is involved in glycogen metabolism and in regulation of muscle contractility. In addition, it is implicated in cell cycle and transcriptional regulation. Recombinant rabbit PP1α has properties in common with the native rabbit muscle protein including size, requirement for Mn²⁺ for activity, specificity for phosphorylase A, and inhibition by okadaic acid, microcystin LR, and phosphatase inhibitor2 (I-2).

Verpackung

Package size based on protein content.

Einheitendefinition

One unit will hydrolyze 1 nmole of p-nitrophenyl phosphate per min at pH 7.4 at 30 °C.

Physikalische Form

Lyophilized powder containing imidazole buffer, pH 7.4, NaCl, DTT, EDTA, MnCl₂, Tween 20, and trehalose as stabilizer.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)