MBD0015
DAPI gebrauchsfertige Lösung
For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL
Synonym(e):
4′,6-Diamidin-2-phenylindol -dihydrochlorid, 2-(4-Amidinophenyl)-6-indolcarbamidin -dihydrochlorid, DAPI -dihydrochlorid
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Alle Fotos(3)
About This Item
Empirische Formel (Hill-System):
C16H15N5 · 2HCl
CAS-Nummer:
Molekulargewicht:
350.25
Beilstein:
4894417
MDL-Nummer:
UNSPSC-Code:
41105500
NACRES:
NA.55
Empfohlene Produkte
Qualitätsniveau
Form
liquid
Lagerbedingungen
protect from light
Konzentration
1 mg/mL
Methode(n)
transfection: suitable
Löslichkeit
water: soluble
Fluoreszenz
λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
Eignung
suitable for nucleic acid staining
Versandbedingung
dry ice
Lagertemp.
−20°C
SMILES String
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChIKey
FPNZBYLXNYPRLR-UHFFFAOYSA-N
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Allgemeine Beschreibung
DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.
Anwendung
DAPI ist um ein Vielfaches empfindlicher als Ethidiumbromid zur DNA-Färbung in Agarosegelen. Es kann für sogenanntes Photo-Footprinting von DNA verwendet werden, zum Nachweis gebundener Sonden in Blotting-Anwendungen durch gezielte Visualisierung des doppelsträngigen Komplexes, als auch um Änderungen in der DNA zu untersuchen und um den DNA-Gehalt während der Apoptose mittels Durchflusszytometrie zu bestimmen. DAPI-Färbung ist eine sensitive und spezifische Nachweismethode für Mykoplasmen.
DAPI Readymade Solution is provided at 1mg/mL concentration. It is recommended to dilute DAPI readymade solution 1:1000 with a desired buffer for a 1μg/mL working concentration (typical working concentration used in most applications is 0.1μg/mL-10μg/mL). Store DAPI solution (1mg/mL) -20°C protected from light. It is recommended to aliquot DAPI readymade solution and to avoid freeze thaw cycles.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS).
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS).
Biochem./physiol. Wirkung
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Leistungsmerkmale und Vorteile
- Ready-to-use liquid, cell permeable dye.
- Can be used with live cells and fixed cells.
- Effective for fixed-cell staining and quantitation of DNA content.
- Optimal Counterstain when using fluorescent antibodies during fluorescence microscopy or high-content screening (HCS) applications.
Physikalische Form
DAPI readymade solution is provided at 1mg/mL concentration in water
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Skin Sens. 1
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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