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Merck

MAK313

Sigma-Aldrich

Adipolysis Assay Kit

sufficient for 200 colorimetric or fluorometric tests

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Anwendung(en)

pharmaceutical

Nachweisverfahren

colorimetric
fluorometric

Relevante Krankheit(en)

endocrinological disorders, diabetes; obesity; cardiovascular diseases; cancer

Lagertemp.

−20°C

Allgemeine Beschreibung

Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. Adipolysis or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery.

Leistungsmerkmale und Vorteile

Compatible with high-throughput handling systems.

Eignung

Suitable for adipolysis quantification in cell culture.

Prinzip

This adipolysis assay kit directly measures glycerol released during adipolysis in cultured cells. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase, and color reactions in one step, resulting in a colorimetric (570 nm)/fluorimetric (λex = 530/ λem = 587 nm) product, proportional to the glycerol present.

Piktogramme

Exclamation mark

Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Acute Tox. 4 Oral

Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

188.6 °F

Flammpunkt (°C)

87 °C


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