L9895

Lectin from Pseudomonas aeruginosa

lyophilized powder

• Synonym(e): PA-I
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00165587
• Form: lyophilized powder

Eigenschaften

• form: lyophilized powder
• Quality Segment: 200
• potency: <15 μg/mL per mL agglutination activity
• storage temp.: −20°C

Beschreibung

Biochem/physiol Actions

PA-I has a major affinity for D-galactose and its derivatives. PA-I agglutinates all types of human and animal erythrocytes and interacts also with unicellular protozoa, algae and bacteria. PA-I induces mitogenic stimulation in cultured human lymphocytes pretreated with neuraminidase.

Packaging

Package size based on protein content. Vial content based on protein by biuret.

Physical form

Lyophilized powder containing approx. 80% protein (biuret), balance primarily sodium citrate, plus traces of calcium chloride, magnesium chloride and manganese chloride

Preparation Note

Purified by affinity chromatography using a modification of Gilboa-Garber.¹

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution using phosphate buffered saline, pH 7.0. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group A erythrocytes after one hr incubation at 25 °C.

Highly purified.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)