L8525

Lipase aus Candida rugosa

lyophilized powder, ≥40,000 units/mg protein

• Synonym(e): Lipase AY 30 Amano, Triacylglycerol-acylhydrolase, Triacylglycerol-lipase
• CAS-Nummer: 9001-62-1
• EC-Nummer: 3.1.1.3 (BRENDA, IUBMB)
• EG-Nummer: 232-619-9
• MDL-Nummer: MFCD00131509
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: fungus (Candida rugosa)
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: ≥40,000 units/mg protein
• Zusammensetzung: Protein, ≥4.0%
• Fremdaktivität: α-amylase ≤0.05 unit/mg solid; protease ≤0.001 units/mg solid
• Lagertemp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChIKey: QWZUIMCIEOCSJF-CHHCPSLASA-N

Beschreibung

Allgemeine Beschreibung

Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Anwendung

Lipasen werden in der Industrie zum Auflösen von chiralen Verbindungen und zur Umesterung bei der Herstellung von Biodiesel verwendet.

Lipase from C. rugosa is used to catalyze the synthesis of flavor esters such as pentyl propanoate and butyl butanoate used in food enhancement.

Lipase from Candida rugosa has been used in a study to assess the influence of phase segregation on enzyme hydrolytic activity.

Lipase from Candida rugosa has been used:

• in the hydrolysis of loaded polycaprolactone–20% tricalcium phosphate (PCL–TCP) composites
• in adsorption of proteins in emulsan/alginate beads and in enzyme assays
• to catalyse methanolysis and hydrolysis reactions

Biochem./physiol. Wirkung

Tri-, Di- und Monoglyceride werden hydrolysiert (in abnehmender Reihenfolge der Rate).

Lipasen katalysieren die Hydrolyse von Triacylglycerinen zu Glycerin und freien Fettsäuren.

Lipases are used in detergent, food, leather, pharmaceutical and diary industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.

Einheitendefinition

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C using olive oil (30 minute incubation).

Hinweis zur Analyse

Protein determined by biuret.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)