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Key Documents

L2018

Sigma-Aldrich

Lipopolysaccharide aus Escherichia coli K-235

purified by gel-filtration chromatography

Synonym(e):

LPS

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About This Item

EG-Nummer:
297-473-0
MDL-Nummer:
MFCD00164401
UNSPSC-Code:
12352201
NACRES:
NA.25

Biologische Quelle

Escherichia coli (K-235)

Qualitätsniveau

200

Form

lyophilized powder

Aufgereinigt durch

gel-filtration chromatography

Verunreinigungen

<1% Protein

Farbe

white to yellow cast

Löslichkeit

water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

This product is extracted from E. coli serotype K235 and purified by gel filtration. The source strain is ATCC 13027.

Biochem./physiol. Wirkung

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Angaben zur Herstellung

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Sonstige Hinweise

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Piktogramme

Skull and crossbones
GHS06

Signalwort

Danger

H-Sätze

H300

Gefahreneinstufungen

Acute Tox. 2 Oral

Lagerklassenschlüssel

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Peipei Zhang et al.
Science advances, 7(43), eabi9471-eabi9471 (2021-10-23)
[Figure: see text].
Waleed Al Abdulmonem et al.
Journal of reproductive immunology, 141, 103152-103152 (2020-06-11)
During first trimester of human pregnancy, the maternal system develops immunity against infection and to provide protection of allogeneic foetus from abortion. This study was undertaken to determine the role of trophoblast specific CD74 isoforms in first trimester trophoblast derived
Okito Hashimoto et al.
Brain, behavior, and immunity, 90, 319-331 (2020-09-21)
Allergic dermatitis (AD), associated with pruritus and itchiness, is one of the major stressful conditions early in life. AD also influences the incidence of neuropsychiatric disorders and developmental disorders through neuro-immune interactions. To the best of our knowledge, there is
Jonathan J Hansen et al.
Inflammatory bowel diseases, 15(6), 890-899 (2009-01-13)
While others have described gene expression patterns in humans with inflammatory bowel diseases and animals with chemically induced colitis, a genome-wide comparison of gene expression in genetically susceptible animals that develop spontaneous colitis has not been reported. We used microarray
Arjen van den Berg et al.
Respiratory research, 6, 111-111 (2005-09-28)
The adenoviral protein E1A has been proposed to play a role in the pathophysiology of COPD, in particular by increasing IL-8 gene transcription of lung epithelial cells in response to cigarette smoke-constituents such as LPS. As IL-8 production is also
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