IPL-41 Insect Medium has been used in culturing silk worm cell lines.[1][2][3]
IPL-41 is part of a series of media originally developed at the USDA Insect Pathology Laboratory by Weiss et al. for the large scale propagation of cells from the fall armyworm, Spodoptera frugiperda. The medium is primarily used for the growth and maintenance of cell lines derived from lepidopterans and the propagation of viruses in these cell lines. One of the most significant uses of the medium is the large scale culture of baculovirus infected Spodoptera cells. The expression of recombinant proteins is undertaken from Spodoptera cells utilizing the baculovirus expression system (BEVS). Attempts have also been made to obtain the expression of foreign genes in cells cultured in IPL-41 without serum supplementation.
Journal of invertebrate pathology, 101(2), 124-129 (2009-05-23)
A new cell line, designated as NIAS-Boma-529b, was established from the larval fat bodies of Bombyx mandarina (B. mandarina), which is believed to be an ancestor of Bombyx mori (B. mori). This cell line has been cultured for approximately 150
Proteasome inhibitor MG132 impairs autophagic flux through compromising formation of autophagosomes in Bombyx cells
Ji M M, et al.
Biochemical and biophysical research communications, 479(4), 690-696 (2016)
Chromatin-induced spindle assembly plays an important role in metaphase congression of silkworm holocentric chromosomes
Mon H, et al.
Insect Biochemistry and Molecular Biology, 45(4), 40-50 (2014)
Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1
Biochemical and biophysical research communications, 479(4), 690-696 (2016-10-04)
MG132 has been used as a proteasome inhibitor on Bombyx cells, but its physiological effects on autophagy still have not been elucidated. In this study, we find that the lipidated BmAtg8, BmAtg8-PE as an autophagosomal marker protein, is only localized
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..