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Wichtige Dokumente
H7787
HIS-Select® Zentrifugationssäulen
Synonym(e):
His-Tag Spin Columns
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About This Item
Empfohlene Produkte
Haltbarkeit
2 yr at 2‑8 °C
Qualitätsniveau
Porengröße
100 nm pore size
Kapazität
>0.5 mg/column
Lagertemp.
2-8°C
Allgemeine Beschreibung
Kit contains 3 collection tubes for every spin column.
Anwendung
Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.
Leistungsmerkmale und Vorteile
- High Selectivity for higher purity
- Convenient and Ready-to-Use
- Consistent protein binding each use
- Compatible with CelLytic™ cell lysis reagents
Rechtliche Hinweise
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Nucleic acids research, 46(14), 7405-7417 (2018-06-16)
Streptomyces are filamentous bacteria with a complex developmental life cycle characterized by the formation of spore-forming aerial hyphae. Transcription of the chaplin and rodlin genes, which are essential for aerial hyphae production, is directed by the extracytoplasmic function (ECF) σ
Molecular microbiology, 84(6), 1033-1049 (2012-05-16)
The chaplin and rodlin proteins together constitute the major components of the hydrophobic sheath that coats the aerial hyphae and spores in Streptomyces, and mutants lacking the chaplins are unable to erect aerial hyphae and differentiate on minimal media. We
Drug design, development and therapy, 10, 3415-3423 (2016-11-02)
Choroidal neovascularization (CNV) in age-related macular degeneration usually causes blindness. We established a novel targeted inhibitor for CNV in age-related macular degeneration. The inhibitor CR2-sFlt 1 comprises a CR2-targeting fragment and an anti-vascular endothelial growth factor (VEGF) domain (sFlt 1).
The Journal of biological chemistry, 285(38), 29147-29155 (2010-07-24)
Three sequential methylations of phosphoethanolamine (PEA) are required for the synthesis of phosphocholine (PCho) in plants. A cDNA encoding an N-methyltransferase that catalyzes the last two methylation steps was cloned from Arabidopsis by heterologous complementation of a Saccharomyces cerevisiae cho2
Journal of bacteriology, 193(16), 4238-4249 (2011-06-07)
Campylobacter jejuni is the leading bacterial cause of human gastroenteritis worldwide. Despite stringent microaerobic growth requirements, C. jejuni is ubiquitous in the aerobic environment and so must possess regulatory systems to sense and adapt to external stimuli, such as oxidative
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