H6536

Monoclonal Anti-HIF-1α antibody produced in mouse

~1 mg/mL, clone H1α67, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Anti-Hypoxia Inducible Factor 1, α subunit
• MDL-Nummer: MFCD01633488
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: H1α67, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 120 kDa
• Speziesreaktivität: rat, sheep, ferret, human, mouse
• Konzentration: ~1 mg/mL
• Methode(n): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500-1:1,000 using antigen retrieval; immunoprecipitation (IP): suitable; western blot: 1:500-1:1,000
• Isotyp: IgG2b
• UniProt-Hinterlegungsnummer: Q16665
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... HIF1A(3091) ; mouse ... Hif1a(15251) ; rat ... Hif1a(29560)

Beschreibung

Allgemeine Beschreibung

Hypoxia-inducible factor-1 (HIF-1) is a 120kD basic helix-loop-helix protein that belongs to the PAS superfamily of transcription factors. It has a crucial role in activating transcription of hypoxia-inducible genes. It can also activate the expression of the gene coding for NIP3; which subsequently induces cell apoptosis under persistent oxygen deprivation. Monoclonal anti-HIF-1α antibody can be used in immunostaining to detect HIF-1α localization. It can also be used in western blotting. Mouse anti-HIF-1α antibody reacts specifically with HIF1 α subunit of sheep, mouse, rat, ferret and human.

Immunogen

peptide corresponding to amino acids 432-528 of the human HIF-1α protein.

Anwendung

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal anti-HIF-1α antibody (diluted 1: 500) can be used in immunofluorescence confocal microscopy. It can also be used in in immunoblotting, immunoprecipitation and immunohistochemistry.

Physikalische Form

Solution in 0.02 M phosphate buffer containing 0.25 M sodium chloride and 0.1% sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable