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GW20071F

Sigma-Aldrich

Anti-β-Galactosidase antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Anti-Beta-Galactosidase

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About This Item

MDL-Nummer:
MFCD00162155
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

chicken

Qualitätsniveau

200

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Speziesreaktivität

bacteria

Hersteller/Markenname

Genway 15-288-20071F

Methode(n)

western blot: suitable

NCBI-Hinterlegungsnummer

NP_752394.1

UniProt-Hinterlegungsnummer

Q8FKG6

Versandbedingung

wet ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Immunogen

Immunogen Sequence: GI # 26246355, sequence 1-1024
Recombinant β-galactosidase [Escherichia coli CFT073]

Anwendung

Anti-β-Galactosidase antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Biochem./physiol. Wirkung

The lac operon (lactose operon) is an essential operon used for the transport and metabolism of lactose in Escherichia coli and some enteric bacteria. It has three adjacent structural genes, lacZ, lacY and lacA. The lacZ gene encodes β-galactosidase. Escherichia coli β-galactosidase is mostly used as a reporter enzyme. It is produced under oxidizing conditions in the cytoplasm of an Origami strain. β-galactosidase occurs in a disulfide bond. At the active site of β-galactosidase a loop opens and closes during catalysis.

Physikalische Form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Rebecca E Harvey et al.
Endocrinology, 158(7), 2179-2189 (2017-04-04)
Ghrelin plays a key role in appetite, energy homeostasis, and glucose regulation. Recent evidence suggests ghrelin suppresses inflammation in obesity; however, whether this is modulated by the acylated and/or des-acylated peptide is unclear. We used mice deficient in acylated ghrelin
Joaquin Seras-Franzoso et al.
Applied and environmental microbiology, 78(7), 2376-2385 (2012-01-31)
Escherichia coli β-galactosidase is probably the most widely used reporter enzyme in molecular biology, cell biology, and biotechnology because of the easy detection of its activity. Its large size and tetrameric structure make this bacterial protein an interesting model for
Larisa J Jancewicz et al.
Archives of biochemistry and biophysics, 517(2), 111-122 (2011-12-14)
A loop (residues 794-803) at the active site of β-galactosidase (Escherichia coli) opens and closes during catalysis. The α and β carbons of Ser-796 form a hydrophobic connection to Phe-601 when the loop is closed while a connection via two

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