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Merck

F2756

Sigma-Aldrich

Fluorescein di(β-D-galactopyranosid)

Synonym(e):

FDG

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About This Item

Empirische Formel (Hill-System):
C32H32O15
CAS-Nummer:
Molekulargewicht:
656.59
MDL-Nummer:
UNSPSC-Code:
12352201
PubChem Substanz-ID:
NACRES:
NC.07

Assay

≥98% (HPLC)

Qualitätsniveau

Form

powder

Löslichkeit

methanol: 20 mg/mL, clear, yellow

Lagertemp.

−20°C

SMILES String

OC[C@H]1O[C@@H](Oc2ccc3c(Oc4cc(O[C@@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)ccc4C36OC(=O)c7ccccc67)c2)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C32H32O15/c33-11-21-23(35)25(37)27(39)30(45-21)42-13-5-7-17-19(9-13)44-20-10-14(43-31-28(40)26(38)24(36)22(12-34)46-31)6-8-18(20)32(17)16-4-2-1-3-15(16)29(41)47-32/h1-10,21-28,30-31,33-40H,11-12H2/t21-,22-,23+,24+,25+,26+,27-,28-,30-,31-/m1/s1

InChIKey

ZTOBILYWTYHOJB-WBCGDKOGSA-N

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Anwendung

Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).
Fluorescein di(β-D-galactopyranoside) has been used as a substrate in β-galactosidase assay.

Biochem./physiol. Wirkung

Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.

Substrate

Fluorogenic substrate for β-galactosidase

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Produkt-Nr.
Beschreibung
Preisangaben

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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