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GLN1

Sigma-Aldrich

Glutamine and Glutamate Determination Kit

Synonym(e):

L-glutamine enzymatic quantification kit

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75

Verwendung

 kit sufficient for 50 tests

Qualitätsniveau

200

Verpackung

pkg of 1 kit

Lagerbedingungen

dry at room temperature

Methode(n)

UV/Vis spectroscopy: suitable

λmax

340 nm

Anwendung(en)

cell analysis
detection

Lagertemp.

2-8°C

Anwendung

For enzymatic determination of L-glutamine and L-glutamate via reduction of NAD to NADH, measured spectrophotometrically at 339 nm.
The detection ranges for this kit are: 0.056 - 0.56 mM L-glutamate; 0.14 - 1.4 mM L-glutamine.
Glutamine and Glutamate Determination Kit has been used to determine the concentration of L-glutamine:
  • in minimum essential medium (MEM) medium
  • in the supernatant
  • in plasma

Biochem./physiol. Wirkung

The Glutamine/Glutamate Determination Kit is designed for the spectrophotometric measurement of L-glutamine and/or L-glutamate via enzymatic deamination of L-glutamine and dehydrogenation of L-glutamate with conversion of NAD+ to NADH. This kit may be used for the spectrophotometric measurement of L-glutamine and/or L-glutamate in liquid preparations.

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Lagerklassenschlüssel

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Wei Shi et al.
Oncology letters, 14(6), 6881-6887 (2017-11-23)
Glycolysis and glutaminolysis are heavily involved in the metabolic reprogramming of cancer cells. The activation of oncogenes and inactivation of tumor suppressor genes has a marked effect on the cellular metabolic processes glycolysis and glutaminolysis. N-Myc downstream-regulated gene 2 (NDRG2)
L-Glutamine and L-glutamate: UV-method with glutaminase and glutamate dehydrogenase.
Lund, P. et al.
Methods of Enzymatic Analysis, 8, 357-363 (1986)
Patricia Martins Bock et al.
Molecular and cellular biochemistry, 411(1-2), 351-362 (2015-11-05)
In this work, we aimed to investigate the effects of long-term supplementations with L-glutamine or L-alanyl-L-glutamine in the high-fat diet (HFD)-fed B6.129SF2/J mouse model over insulin sensitivity response and signaling, oxidative stress markers, metabolism and HSP70 expression. Mice were fed
Inhibition of Alanine Aminotransferase in Silico and in Vivo Promotes Mitochondrial Metabolism to Impair Malignant Growth
Beuster G, et al.
The Journal of biological chemistry, 286(25), 22323-22330 (2011)
Monitoring for overreaching in rugby league players
Coutts AJ, et al.
European Journal of Applied Physiology, 99(3), 313-324 (2007)
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