GE17-0963-03
Protein A Sepharose™ Cl-4B
Cytiva 17-0963-03, pack of 25 mL
Synonym(e):
Protein A Column, Sepharose 4B
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About This Item
UNSPSC-Code:
41106500
NACRES:
NA.56
Empfohlene Produkte
ligand
native protein A
Verpackung
pack of 25 mL
Hersteller/Markenname
Cytiva 17-0963-03
Lagerbedingungen
(20% Ehtanol)
Matrix
4% cross-linked agarose
Partikelgröße
45-165 μm
Durchschnittlicher Durchmesser
90 μm
cleaning
2-10
Arbeitsbereich
3-9
Kapazität
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
Lagertemp.
2-8°C
Allgemeine Beschreibung
Protein A Sepharose™ CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Leistungsmerkmale und Vorteile
- Well documented for immunoglobulin purification and fractionation
- Strong binding to Fc region of IgG
- High capacity - 20 mg human IgG/mL.
- Suited for immunoprecipitation procedures
Lagerung und Haltbarkeit
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Hinweis zur Analyse
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Rechtliche Hinweise
Sepharose is a trademark of Cytiva
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
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