G0407

Granulocyte colony-stimulating factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• MDL-Nummer: MFCD00166475
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• product name: Granulocyte colony-stimulating factor human, G-CSF, recombinant, expressed in E. coli, suitable for cell culture
• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: 0.01-0.1 ng/mL
• Qualität: endotoxin tested
• Mol-Gew.: 18.8 kDa (175 amino acids including N-terminal methionine)
• Verpackung: pkg of 5 and 25 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: <1 EU/μg (LAL test)
• UniProt-Hinterlegungsnummer: P09919
• Lagertemp.: −20°C
• Angaben zum Gen: human ... CSF3(1440)

Beschreibung

Allgemeine Beschreibung

Granulocyte colony-stimulating factor human (GCSF) gene is mapped to human chromosome 17q21.1. Structurally, GCSF comprises a four α-helical bundle.

Anwendung

Granulocyte colony-stimulating factor human has been used:

• in culturing human neutrophils
• as a growth factor component of double layer granulocyte-macrophage colony-forming unit (CFU-GM) culture system for CD34⁺ cells
• to test its protective effects chronic liver damage model

Biochem./physiol. Wirkung

Granulocyte colony-stimulating factor human (GCSF) interacts specifically with the immunoglobin (Ig) domain of the granulocyte colony-stimulating factor receptor (GCSFR).

Granulocyte-colony stimulating factor (G-CSF) is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G₀ period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.

Physikalische Form

Lyophilized from 0.2 μm-filtered solution of 10 mM HOAc containing 50 μg BSA per μg G-CSF

Hinweis zur Analyse

The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)