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F3046

Sigma-Aldrich

Monoklonales Anti-Aktin

clone AC-40, purified immunoglobulin, buffered aqueous solution

Synonym(e):

Monoclonal Anti-Actin

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

200

Konjugat

FITC conjugate

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

AC-40, monoclonal

Form

buffered aqueous solution

Mol-Gew.

antigen 42 kDa

Speziesreaktivität

human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat

Methode(n)

direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts

Isotyp

IgG2a

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... ACTA1(58) , ACTA1(58) , ACTA1(58) , ACTA2(59) , ACTA2(59) , ACTA2(59) , ACTB(60) , ACTB(60) , ACTB(60) , ACTC1(70) , ACTC1(70) , ACTC1(70) , ACTG1(71) , ACTG1(71) , ACTG1(71) , ACTG2(72) , ACTG2(72) , ACTG2(72)
mouse ... Acta1(11459) , Acta1(11459) , Acta1(11459) , Acta2(11475) , Acta2(11475) , Acta2(11475) , Actb(11461) , Actb(11461) , Actb(11461) , Actc1(11464) , Actc1(11464) , Actc1(11464) , Actg1(11465) , Actg1(11465) , Actg1(11465) , Actg2(11468) , Actg2(11468) , Actg2(11468)
rat ... Acta1(29437) , Acta1(29437) , Acta1(29437) , Acta2(81633) , Acta2(81633) , Acta2(81633) , Actb(81822) , Actb(81822) , Actb(81822) , Actc1(29275) , Actc1(29275) , Actc1(29275) , Actg1(287876) , Actg1(287876) , Actg1(287876) , Actg2(25365) , Actg2(25365) , Actg2(25365)

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Allgemeine Beschreibung

Der Antikörper erkennt ein Epitop am C-Terminus von Aktin, der in allen Aktin-Isoformen konserviert ist. Er markiert gezielt Aktin in einer Vielzahl von Geweben und Spezies. Das vom Antikörper erkannte Epitop ist resistent gegenüber Formalinfixierung und Paraffineinbettung. Zink-Formalin, B5, Ethanol, Methacarn und Brunnel- oder Bouin-Lösung können ebenfalls als Fixiermittel verwendet werden.
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. It has one N-terminal region which appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

Immunogen

Synthetisches C-terminales Aktin-Peptid, das sich am Rückgrat von multiplen Antigenpeptiden (MAP) befindet

Anwendung

Als primärer Antikörper für die Western-Blot-Analyse von MDCK-Zelllysaten wurde ein monoklonaler Anti-Aktin-Antikörper verwendet.
Monoklonaler Anti-Aktin-Antikörper wurde für die Western-Blot-Analyse von Cos-7-Zelllysaten verwendet, um eine gleichmäßige Beladung von Proteinen zu garantieren.
Actin–FITC antibody is suitable for direct immunofluorescence at a dilution of 1:75, using cultured human or chicken fibroblasts.

Biochem./physiol. Wirkung

Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. It is associated with myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. In mammals and birds, actin is expressed as six different isoforms. It has one N-terminal region which appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Sonstige Hinweise

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

J L Lessard
Cell motility and the cytoskeleton, 10(3), 349-362 (1988-01-01)
Two IgG1, kappa monoclonal antibodies (Mab) against actin have been obtained from a fusion in which chicken gizzard actin was used as the immunogen. One Mab, designated B4, shows a preferential reactivity toward enteric smooth muscle actin but also cross-reacts
K Sutoh et al.
Biochemistry, 25(20), 6186-6192 (1986-10-07)
An antibody was raised against the N-terminal 18 residues of rabbit skeletal muscle actin. By the use of this antibody as the N-terminal probe of actin and the fluorescent label at Cys-374 as its C-terminal probe, binding sites of depactin
Kimberly A Moore et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 232(2), 268-281 (2004-12-23)
Local alterations in the mechanical compliance of the basement membrane that alter the level of isometric tension in the cell have been postulated to influence tissue morphogenesis. To explore whether cell tension contributes to tissue pattern formation in vivo, we
I M Herman
Current opinion in cell biology, 5(1), 48-55 (1993-02-01)
The actin supergene family encodes a number of structurally related, but perhaps functionally distinct, protein isoforms that regulate contractile potential in muscle tissues and help to control the shape as well as the motility of non-muscle cells. In spite of
J S Drew et al.
The American journal of physiology, 260(6 Pt 1), C1332-C1340 (1991-06-01)
Smooth muscle cells contain both smooth muscle-specific and cytoplasmic actin isoforms. We tested the hypothesis that these two classes of isoactins are segregated into separate "contractile" vs. "cytoskeletal" populations of thin filaments. Isolated thin filaments from adult swine stomach were
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