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D5921

Sigma-Aldrich

DMEM - low glucose

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Synonym(e):

DME, DMEM

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About This Item

MDL-Nummer:
MFCD00217342
UNSPSC-Code:
12352207
NACRES:
NA.75

product name

Dulbecco′s Modified Eagle′s Medium, niedriger Glucosegehalt, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Qualitätsniveau

400

Sterilität

sterile-filtered

Form

liquid

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

endotoxin, tested

Komponenten

phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO3: yes

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME), which has a four-fold higher concentration of amino acids and vitamins along with additional supplementary components. The first original DME formula was reported to culture embryonic mouse cells comprising 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. DME has been shown to improve the stability of the medium.

Anwendung

Dulbecco′s Modified Eagle′s medium-low glucose has been used to culture:
  • living colon cancer SW620 cells
  • human breast carcinoma cell lines MCF-7(13), KPL and MDA-MB-231
  • mammal and canine derived adipose-derived mesenchymal stem cells

Rekonstituierung

Supplement with 0.584 g/L L-glutamine.

Sonstige Hinweise

Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

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Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H317

Gefahreneinstufungen

Skin Sens. 1

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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