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D2429

Sigma-Aldrich

DMEM - low glucose

With sodium pyruvate, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable fore cell culture

Synonym(e):

DME, DMEM

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About This Item

MDL-Nummer:
MFCD00217342
UNSPSC-Code:
12352207
NACRES:
NA.75

Produktbezeichnung

Dulbecco′s Modified Eagle′s Medium, niedriger Glucosegehalt, 10 ×, With 1000 mg/L glucose (1x), without L-glutamine, sodium bicarbonate, and folic acid, liquid, sterile-filtered, suitable for cell culture

Qualitätsniveau

400

Sterilität

sterile-filtered

Form

liquid

Konzentration

10 ×

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

endotoxin, tested

Komponenten

NaHCO3: no
sodium pyruvate: yes
phenol red: yes
glucose: low
L-glutamine: no
HEPES: no

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Dulbecco′s Modified Eagle′s Medium (DME) is a modification of basal medium Eagle (BME) that contains a 4-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose.

Anwendung

Dulbecco′s Modified Eagle′s Medium-low glucose has been used:
  • during organoid embedding for neutralizing thawed collagen I
  • to culture melanoma cancer cell lines
  • to store the cancer tissue samples
  • as a differentiation culture medium for C2C12 cells

Rekonstituierung

Supplement with 3.7 g/L sodium bicarbonate, 0.584 g/L L-glutamine, 0.004 g/L folic acid, at 1×.

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Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H317

Gefahreneinstufungen

Skin Sens. 1

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
RNBL7902
RNBK8505
RNBJ9318
RNBJ4442
RNBJ2213

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