Direkt zum Inhalt
Merck
Alle Fotos(1)

Wichtige Dokumente

Gesamtdokumentation anzeigen

CGP1

Sigma-Aldrich

Glutathione Peroxidase Cellular Activity Assay Kit

Sufficient for 100 colorimetric tests

Synonym(e):

Cellular glutathione peroxidase, c-GPx

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

UNSPSC-Code:
12161503
NACRES:
NA.32

Qualitätsniveau

200

Verwendung

sufficient for 100 tests

Haltbarkeit

Kit is stable for 24 months when unopened.

Nachweisverfahren

colorimetric

Versandbedingung

wet ice

Lagertemp.

−20°C

Allgemeine Beschreibung

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Eignung

Suitable for the measureent of glutathione peroxidase in tissue extracts

Prinzip

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Sonstige Hinweise

This kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung
  • tert-Butyl hydroperoxide 1 mL

Ähnliches Produkt

Produkt-Nr.
Beschreibung
Preisangaben

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

5.2 - Organic peroxides and self-reacting hazardous materials

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

International Committee for Standardization in Haematology: recommended methods for red-cell enzyme analysis.
E Beutler et al.
British journal of haematology, 35(2), 331-340 (1977-02-01)
I Mavelli et al.
European journal of biochemistry, 139(1), 13-18 (1984-02-15)
Red blood cells of favism patients with acute hemolytic crisis have markedly more superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1) and less glutathione peroxidase (glutathione:hydrogenperoxide oxidoreductase, EC 1.11.1.9) than either normal controls, glucose-6-phosphate dehydrogenase-deficient subjects or favism patients outside hemolytic crisis.
Diversity of glutathione peroxidases.
F Ursini et al.
Methods in enzymology, 252, 38-53 (1995-01-01)
Ian M Scott et al.
Pesticide biochemistry and physiology, 141, 9-17 (2017-09-16)
Plant elicitors can be biological or chemical-derived stimulators of jasmonic acid (JA) or salicylic acid (SA) pathways shown to prime the defenses in many crops. Examples of chemical elicitors of the JA and SA pathways include methyl-jasmonate and 1,2,3-benzothiadiazole-7-carbothioate (BTH
Matthias L Jauslin et al.
Human molecular genetics, 11(24), 3055-3063 (2002-11-06)
Friedreich Ataxia (FRDA), the most prevalent of the inherited ataxias, is a multi-systemic disease with loss of sensory neurons and life-threatening hypertrophic cardiomyopathy as its most severe manifestations. Reduced levels of the mitochondrial protein frataxin lead to cell-damaging oxidative stress
Alle zugehörigen Dokumente anzeigen

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.