C8696

Cathepsin D from human liver

lyophilized powder, ≥250 units/mg protein (E1%/280)

• CAS-Nummer: 9025-26-7
• EC-Nummer: 3.4.23.5 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00130749
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Form: lyophilized powder
• Qualitätsniveau: 200
• Spezifische Aktivität: ≥250 units/mg protein (E1%/280)
• Mol-Gew.: ~45 kDa
• Farbe: white
• UniProt-Hinterlegungsnummer: P07339
• Lagertemp.: −20°C
• Angaben zum Gen: human ... CTSD(1509)

Beschreibung

Allgemeine Beschreibung

Cathepsin D is an aspartic protease, which is located in lysosomes. It is involved in protein catabolism and maintains hormone and antigen processing. Cathepsin D is implicated in neoplasia and neurodegenerative changes. It regulates lysosomal proteolysis and endogenous fibrinolysis.[1]

Anwendung

Cathepsin D from human liver has been used:

• in β-secretase activity assay[2]
• for enzymatic degradation of amyloid β 1-42[3]
• in microinjection of human foreskin fibroblasts[4]

Biochem./physiol. Wirkung

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.

Endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts.

Sonstige Hinweise

Contains α, β and γ isoenzymes as observed by isoelectric-focusing.

Einheitendefinition

One unit will produce an increase in A₂₈₀ of 1.0 in 30 min at pH 3.3 at 37 °C measured as TCA-soluble products using acid-denatured hemoglobin as substrate (1 cm light path).

Physikalische Form

Powder containing sodium phosphate buffer salt.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable