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Merck

C8210

Sigma-Aldrich

CHES

BioXtra, ≥99.0% (titration)

Synonym(e):

2-(Cyclohexylamino)-ethansulfonsäure

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About This Item

Empirische Formel (Hill-System):
C8H17NO3S
CAS-Nummer:
Molekulargewicht:
207.29
Beilstein:
2967601
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12161700
PubChem Substanz-ID:
NACRES:
NA.25

Produktlinie

BioXtra

Qualitätsniveau

Assay

≥99.0% (titration)

Form

powder

Verunreinigungen

≤0.0005% Phosphorus (P)
≤0.1% Insoluble matter

Glührückstand

≤0.1%

pH-Wert

3.0-5.0 (0.5 M in H2O)

Nützlicher pH-Bereich

8.6-10.0

pKa (25 °C)

9.3

Löslichkeit

H2O: 0.5 M, clear, colorless

Anionenspuren

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

Kationenspuren

Al: ≤0.0005%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.01%
Pb: ≤0.001%
Zn: ≤0.0005%

Absorption

≤0.05 at 280 in H2O at 0.5 M
≤0.08 at 260 in H2O at 0.5 M

Anwendung(en)

diagnostic assay manufacturing

SMILES String

OS(=O)(=O)CCNC1CCCCC1

InChI

1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)

InChIKey

MKWKNSIESPFAQN-UHFFFAOYSA-N

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Allgemeine Beschreibung

CHES (2-(Cyclohexylamino) ethane sulfonic acid) is a derivative of amino acid taurine. It serves as a standard physiological buffer in the pH range of 8.5-9.5.

Anwendung

CHES may be used:
  • for pH adjustments and as a assay buffer in catechol oxidase activity measurements(32)
  • as a buffer in electron paramagnetic resonance (EPR) spectroscopy and kinetic measurements(33)
  • as a buffer for soil solution equivalent (SSE) yeast dextrose medium for screening pH optima for growth of Aridibacter nitratireducens A24_SHP-5_238T(34)

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Gloves


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Kunden haben sich ebenfalls angesehen

Buffers for the physiological pH range: Acidic dissociation constants of zwitterionic compounds (ACES and CHES) in water from 5 to 55? C
Roy RN, et al.
Journal of Chemical and Engineering Data, 42(1), 41-44 (1997)
P Doble et al.
Electrophoresis, 19(12), 2257-2261 (1998-10-07)
The suitability of relatively slow (low absolute value of mobility) coanionic buffers in background electrolytes (BGEs) for indirect photometric detection of anions by capillary electrophoresis was investigated. As a model system, 2-(cyclohexylamino)ethanesulfonic acid (CHES) was used to buffer the indirect
Scott A Sarver et al.
Journal of chromatography. A, 1229, 268-273 (2012-02-11)
Several glycosphingolipids were labeled with the fluorphore Bodipy-Fl and analyzed using capillary electrophoresis with laser-induced fluorescence detection. GM1-, LacCer-, and Cer-Bodipy-Fl were prepared through acylation using the N-hydroxysuccinimide ester of Bodipy-Fl. Several other glycosphingolipids including GT1a-, GD1a-, GM2-, GM3-, GD3-
David G McLaren et al.
Electrophoresis, 24(17), 2887-2895 (2003-09-16)
A systematic evaluation of continuous flow-counterbalanced capillary electrophoresis for microscale preparative applications is presented. The success of the technique was found to depend on the specific nature of the analyte and background electrolyte and was most heavily influenced by factors
L M Gloss et al.
Biochemistry, 34(12), 3999-4007 (1995-03-28)
The pH dependence of Escherichia coli aspartate aminotransferase (AATase) has been investigated by the use of site-directed mutants and alternative substrates. Inhibition of the enzyme by CHES and variations in ionic strength are proposed to explain some of the qualitative

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