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Merck

C5338

Sigma-Aldrich

Cyanogenbromid-aktivierte Sepharose 4 Fast Flow

lyophilized powder

Synonym(e):

Cyanogenbromid-aktivierte Agarose

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About This Item

CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
41106500
NACRES:
NA.56

Form

lyophilized powder

Qualitätsniveau

Methode(n)

affinity chromatography: suitable

Matrix

Sepharose 4 Fast Flow

Aktive Matrixgruppe

cyanate or related structures

Matrix-Spacer

1 atom (when ligands are coupled via isourea derivative or related linkage)

Quellen

1 g swells to 4-5 mL gel

Partikelgröße

45—165 μm

Kapazität

6-11 μmol/mL coupling capacity (6-AKS)

Lagertemp.

2-8°C

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Allgemeine Beschreibung

C5338-10G′s update product number is GE17-0981-01
Cyanogen bromide-activated-Sepharose® 4 Fast Flow is a resin composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. Proteins and other molecules containing primary amino groups are coupled directly to the preactivated gel. It allows for multi-point attachment of protein ligands which minimizes ligand leakage. The resin is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.

Anwendung

Cyanogen bromide-activated-Sepharose is used in affinity chromatography and protein chromatography. Cyanogen bromide-activated-Sepharose has been used to discover insecticidal agents, to raise polyclonal antibodies against the yeast invertase glycosyls, and to study immunological processes of xenograft rejection.

Physikalische Form

Lyophilisiertes Pulver stabilisiert mit Zucker-Stabilisatoren

Rechtliche Hinweise

Sepharose is a trademark of Cytiva

Ersetzt durch

Produkt-Nr.
Beschreibung
Preisangaben

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Transplantation proceedings, 38(10), 3312-3314 (2006-12-19)
Human natural killer (NK) cells, which can mediate direct lysis of porcine endothelial cells, play an important role in xenograft rejection. HLA-G, which is a critical molecule in maintaining maternal immune tolerance of semi-allogenic fetus, is able to protect susceptible
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Biotechnology and bioengineering, 56(6), 605-609 (2008-07-22)
Polyclonal antibodies directed against the yeast invertase glycosyls were raised by immunizing rabbits with neoglycoprotein-I and neoglycoprotein-II. The neoglycoproteins were prepared by separately coupling the N-linked large and small molecular weight yeast invertase oligosaccharides respectively to bovine serum albumin with
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